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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL50 value of greater than 320 mg/L loading rate WAF.  The No Observed Effect Loading rate was 320 mg/L loading rate WAF.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
320 mg/L

Additional information


A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.


Following initial tests, twenty daphnids (2 replicates of 10 animals) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 3.2, 10, 32, 100 and 320 mg/L for 48 hours at a temperature of 22°C under static test conditions. The number of immobilized Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.


The 48-Hour EL50 (Effective Loading Rate)for the test item to Daphnia magna based on nominal loading rates was greater than 320 mg/L loading rate WAF. The No Observed Effect Loading rate was 320 mg/L loading rate WAF.

Chemical analysis of the 320 mg/L loading rate WAF at 0 and 48 hours showed measured test concentrations of 0.0515 mg/L and less than the limit of quantitation (LOQ) of the analytical method employed were obtained which was determined to be 0.00012 mg/L respectively. Given the extremely low aqueous solubility of the test item and the resultant low initial measured concentration, the decline in measured concentration over the test period was not taken as any evidence of instability of the test item.

The dissolved test item may have been one or several components of the test item. Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.