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EC number: 265-747-9 | CAS number: 65405-84-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6.01. - 28.03.2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline under GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- α,2,2,6-tetramethylcyclohexene-1-butyraldehyde
- EC Number:
- 265-747-9
- EC Name:
- α,2,2,6-tetramethylcyclohexene-1-butyraldehyde
- Cas Number:
- 65405-84-7
- Molecular formula:
- C14H24O
- IUPAC Name:
- 2-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)butanal
- Details on test material:
- - Name of test material (as cited in study report):
- Physical state: liquid
- Storage condition of test material: At room temperature (at about 20 °C), in a tightly closed container
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: duplicate samples were taken before start and after 72 hours from both the control and the test samples
- Sample storage conditions before analysis: All samples were deep-frozen (at about -20 °C) immediately after sampling.
Test solutions
- Details on test solutions:
- The test item (64.96 mg) was dissolved in iso-octane and made up to the mark in a 50 mL
volumetric flask to prepare a stock solution of 1299.2 mglL. Defined volumes of this stock
solution were diluted with iso-octane to obtain standard solutions of the test item in the range
of 0.650 to 130 mg/L. These solutions were used to calibrate the GC-system.
Treatment samples and control samples were thawed at 35" C (water bath) for two hours and
shaken manually to obtain homogeneous -- -- . sample solutions.
The samples were extracted two times with 100 mL of pentan. After addition of 2 mL isooctane
(keeper) the combined organic phases were evaporated to 2 mL using a rotary
evaporator (20 minutes in a 35°C water bath). The 2mL were transferred in a 20 mL
volumetric flask and made up to the mark with iso-octane.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: No. 86.81 SAG
- Source (laboratory, culture collection): (SAG, Institute for Plant Physiology, University of Göttingen, Germany
ACCLIMATION
- Culturing media and conditions (same as test or not): same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- not applicable
Test conditions
- Hardness:
- 0.24 mmol/L (= 24 mg/L) as CaC03.
- Test temperature:
- 22-23°C
- pH:
- 8.2 - 8.8
- Dissolved oxygen:
- no data
- Salinity:
- -
- Nominal and measured concentrations:
- nominal loading rate 100 mg/L, mean measured concentration 1.76 mg/L
- Details on test conditions:
- The test was started (0 hours) by inoculation of 10,000 algal cells per mL of test medium.
These cells were taken from an exponentially growing pre-culture that was set up four days
prior to the test under the same conditions as in the test. One day before the start of the test,
the pre-culture was diluted threefold to keep the algae in exponential growth.
The test design included three replicates of the single test concentration and six replicates of
the control. Volumes of 15 mL algal suspension for each replicate were continuously stirred by
magnetic stirrers in 50 mL Erlenmeyer flasks.
The flasks were covered with glass dishes. They were incubated in a temperature controlled
water bath at a temperature of 22-23 "C (Table 4), and continuously irradiated at a measured
light intensity of about 7300 Lux (mean value), range: 6610 to 7930 Lux (minimum and
maximum value of measurements at nine places distributed over the experimental area at the
surface of the test media). This illumination was achieved by fluorescent tubes, installed about 35 cm above the test flasks.
The test duration was 72 hours. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: The mean algal cell density in the test medium was at all counting dates slightly higher than
that in the parallel control cultures
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
: - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 72-h EC50 for the growth rate: 0.78 mg/L - Reported statistics and error estimates:
- A statistical evaluation of the algal cell densities was not necessary since the mean algal cell
density in the test medium was at all counting dates similar to or even slightly higher than in
the control.
Any other information on results incl. tables
No remarks
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, Dihydro-C14-Aldehyde and its possible degradation products had no toxic effect
on the algae up to their solubility limit in test water under the present test conditions. - Executive summary:
The influence of the test item Dihydro-C14-Aldehyde on the growth of the green algal species
Scenedesmus subspicatus was investigated in a 72-hour static test according to the EU
Commission Directive 92/69/EEC, C.3, 1992, and the OECD Guideline No. 201, 1984).
Due to the low water solubility of the test item, a supersaturated dispersion of the test item with
a loading rate of 100 mg/L was continuously stirred at room temperature in the dark over three
hours. After the stirring period an oily film was present on the surface. Therefore, only a part of
the dispersion of the test item was taken out of the middle of the water column and filtered
through a membrane filter just before the preparation of the test media. This procedure was necessary to avoid a penetration, of the
oily film through the filter into the test medium. The undiluted filtrate with the maximum
concentration of dissolved test item was used as the only test medium. Additionally, a control
was tested in parallel. Thus, a limit test was performed according to the EU Commission
Directive 92169/EEC.
The test item concentration in the analyzed test medium samples amounted to 2.45 mg/L at
the start of the test. In this test medium, incubated under the conditions of the test during the
test period (but without algae), a decrease of the test item concentration was determined. At
the end of the test, a test concentration of 1.26 mg/L was found. This decrease of the
measured test item concentration was considered to be caused by a loss by adsorption onto
the glass surfaces or by degradation.
The biological results are based on the test item concentration of 1.76 mg/L (calculated as the
average over all measurements in the undiluted filtrate) and on the loading rate of 100 mg/L.
The test item Dihydro-C14-Aldehyde and its possible degradation products had clearly no
inhibitory effect on the growth of Scenedesmus subspicatus during the test period of 72 hours
at the loading rate of 100 mg/L (mean measured concentration of 1.76 mg/L).
The 72-hour NOEC (highest concentration tested without toxic effects after a test period of
72 hours) was determined to be at the loading rate of 100 mg/L. The NOEC might even be
higher but test item concentrations above the solubility limit of the test item in test water or
loading rates above 100 mg/L were not tested, according to the EU Commission Directive
92169/EEC. The 72-hour LOEC (lowest concentration tested with toxic effects after a test
period of 72 hours), and the 72-hour EC10 and EC50 were clearly higher than the loading rate
of 100 mg/L. These values could not be quantified, since the test item had no toxic effect on
the algae up to the highest test item concentration which could be dissolved in the test water
at a loading rate of 100 mg/L.
In conclusion, Dihydro-C14-Aldehyde and its possible degradation products had no toxic effect
on the algae up to their solubility limit in test water.
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