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EC number: 248-368-3 | CAS number: 27253-26-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study report does not state whether testing was performed under Good Laboratory Practices (GLP).
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Phthalate Ester Testing In The National Toxicology Program's Environmental Mutagenesis Test Development Program
- Author:
- Zeiger E, Haworth S, Speck W and Mortelmans K
- Year:
- 1 982
- Bibliographic source:
- Environmental Health Perspectives, Volume 45, Pages 99-101
- Reference Type:
- publication
- Title:
- Mutagenicity testing of di(2-ethylhexyl) phthalate and related chemicals in salmonella
- Author:
- Zeiger E, Haworth S, Mortelmans K and Speck W
- Year:
- 1 985
- Bibliographic source:
- Environmental Mutagenesis, Volume 7, Pages 213-232
Materials and methods
Test guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- A mutagenic response was defined as a reproducible, dose-related increase in the number of histidine-independent colonies over the spontaneous incidence. There was no requirement for a specific magnitude of increase.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Di(tridecyl) phthalate
- EC Number:
- 204-294-3
- EC Name:
- Di(tridecyl) phthalate
- Cas Number:
- 119-06-2
- IUPAC Name:
- ditridecyl phthalate
- Details on test material:
- All testing was done using a preincubation modification of the method of Ames, Salmonella typhimurium TA98, TA100, TA1535 and TA1537 were used with S-9 liver homogenate preparations from Arochlor 1254-induced male Sprague-Dawley rats and Syrian hamsters. An overnight culture of Salmonella, S-9 fraction and DTDP were plated onto minimal agar at 37 Celsius for two days, after which the histidine revertant colonies were machine counted. DTDP was tested at 5 different concentrations.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction of Aroclor 1254 induced rat and hamster liver
- Test concentrations with justification for top dose:
- 5 dose levels up to 10 mg/plate
- Details on test system and experimental conditions:
- Approximately 10E8 bacteria were mixed with 0.5 ml of either 0.1M sodium phosphate buffer or S-9 mix, and test substance. The reaction was carried out in triplicate. The mixture was incubated at 37°C for 48 hours, after which time histidine-revertant colonies were counted. The doses selected were separated by half-log intervals. The high dose was 10 mg/plate unless limited by solubility. Positive control chemicals were sodium azide, nitro-o-phenylenediamine, 9-aminoacridine and 2-aminoanthracene. Concurrent solvent and positive controls were included in all experiments. A toxicity pretest with TA 100 was conducted to determine the high dose level.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- No mutagenic activity was observed at doses up to 10 mg/plate in Salmonella strains TA 98, TA 100, TA 1535 and TA 1537 with or without metabolic activation.
- Executive summary:
The mutagenic capacity of DTDP was assessed using the Ames method. Salmonella typhimurium TA98, TA100, TA1535 and TA1537 were used with S-9 liver homogenate preparations from Arochlor 1254-induced male Sprague-Dawley rats and Syrian hamsters. An overnight culture of Salmonella, +/- S-9 fraction and DTDP were plated onto minimal agar at 37 Celsius for two days, after which the histidine revertant colonies were machine counted. Five different concentrations of DTDP were used. No mutagenic activity was observed at doses up to 10 mg/plate in Salmonella strains TA 98, TA 100, TA 1535 and TA 1537 with or without metabolic activation.
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