Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 401-620-8 | CAS number: 87731-18-8 VIOLIFF
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The substance is readily biodegradable and shows 67% degradation in 28 days in an OECD TG 301D test.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The biodegradability of the substance was tested in a Closed Bottle Test according to OECD 301D and in compliance with GLP. Test substance at a concentration of ca. 2 mg/L was inoculated with activated sewage sludge from an STP treating predominantly domestic sewage for 28 days. At inoculation days, 0, 5, 15 and 30, the oxygen consumption was measured and biodegradation determined as % of ThOD. Biodegradation of the substance started directly at day 0 and increased up to 67% at day 15. No further degradation was found thereafter. Although the study design does not allow to determine whether or not the 14 -day window was met, based the rapid biodegradation to a value of well above the cut-off criterion of 60% at day 15, it is considered justified to conclude that the substance is readily biodegradable.
For Violiff there are three screening studies available. The Closed Bottle Test is presented above. There is a Respirometric Method (modified MITI Test I) according to OECD 301C (IFF, 2015). In this test Violiff showed only minor biodegradation on day 28 (8% based on BOD and 7% based on DOC). Mean decrease of test material (via GC analysis) on day 28 was 55%. Several alcohol and ketone metabolites were identified. In a Manometric Respirometry Test according to OECD 301F (Givaudan, 2011) the test substance tested at 30 mg/L did undergo 60% biodegradation after 28 days (73% after 50 days).
The available data on biodegradation are all considered valid studies. In this case positive results can be considered valid, irrespective of negative results, when guideline criteria are fulfilled. Although the pass window was not fulfilled in the OECD 301F and could not be assessed in the OECD 301D it has to be noted that the substance is a mixture and therefore degradation percentage should be evaluated at the end of the test period. In both the OECD 301D and 301F the degradation percentage reached was equal or higher to 60% after 28 days. Both studies should be interpreted as readily degradable and therefore meet the criterion rapidly degradable under GHS.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
