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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Section 2
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Code number : FAT - 92'368/A
EN/Batch : PLN 13
Purity : ca 80%
Appearance : solid
Solubility : miscible
Storage : room temperature
Expiration date : 03/98
Analytical monitoring:
yes
Details on sampling:
Samples for analytical determinations were taken at 0 and 48 h after mixing of the test solutions from the approximate center of the test vessels. The samples were stored at -18 to - 25 °C until analyses.
Vehicle:
yes
Details on test solutions:
Stock solution:
0.5 g test substance was mixed with 200 mL test medium and the volume was then made up to 500 mL with test medium. The stock solution was homogenized for five minutes in an ultrasonic bath.
Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia were bred in the testing facility of the Product Ecology TF, Textile Dyes Division under standardised conditions.

BREEDING:
Cultures of Daphnia magna were maintained in glass vessels containing approx. 3.0 L of reconstituted water at 18-22 °C. The water was renewed partially three times weekly. The Daphnia were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food was consumed within 24 h. The quality of the Daphnia was checked every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8-1.5 mg/L).

PRETREATMENT :
24 h before the start of the exposure, reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 710 µm sieve.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
none
Post exposure observation period:
no data
Hardness:
136 mg/L CaCO3 (theoretical water hardness)
Test temperature:
21.6 - 21.8 °C
pH:
7.8 - 8.0
Dissolved oxygen:
The medium was oxygen saturated at the beginning of the test. The oxygen concentrations ranged from 96 - 99 % through the study duration.
Salinity:
no data
Nominal and measured concentrations:
Nominal: 10, 18, 32, 58, 100 and 180 mg/L (test concentrations chosen on the basis of a pre-test).
Measured: 9.2, 17, 32, 55, 98, 175 mg/L (at 0 h) and 9.1, 18, 32, 57, 100 and 175 mg/L (at 48 h).
Average of measured concentrations: 9.2, 18, 32, 56, 99 and 175 mg/L.

During the test duration, the test article concentrations in the analysed test media were in the range of 91.0-100.0% of the nominal values. In all test concentrations non alteration of the test substance after 48 h was observed. Therefore, under the test conditions the test article was sufficiently stable.
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL beakers containing 100-200 mL test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Test medium composition: NaHCO3 = 65 mg/L; MgSO4 * 7H2O = 123.3 mg/L; KCl = 5.8 mg/L; CaCI2 * 2H2O = 294 mg/L; Na2SiO3 * 9H2O = 10 mg/L; NaNO3 = 274 µg/L; KH2PO4 = 143 µg/L; K2HPO4 = 184 µg/L
- Dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4: 1
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Photoperiod: 16 h illumination.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 9.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 47.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: The EC50 value was calculated with the " Probit model " method. 95% confidence limit (24h): 38.73 - 57.96
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
175 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 9.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
30.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: The EC50 value was calculated with the " Probit model " method. 95% confidence limit (48h): 26.21 - 35.87
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
56 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 9.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations.
Results with reference substance (positive control):
Potassium dichromate 24 h EC50 = 0.97 mg/L
Reported statistics and error estimates:
no data

Table 1: Immobilization of Daphnia

Average measured concentration of

test substance

(mg/L)

 

No. of

Animals

% immobility

24 h

48 h

Blank

20

0

0

9.2

20

0

0

18

20

10

10

32

20

10

45

56

20

80

100

99

20

80

100

175

20

100

-

 

Validity criteria fulfilled:
yes
Conclusions:
The test substance is slightly toxic to Daphnia with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h respectively.
Executive summary:

A study was conducted to determine the acute toxicity of the test substance FAT 92368/A to Daphnia magna in a 48 h static test according to the OECD Guideline 202, Section 2, and the EU Method C.2, in GLP compliance.

Daphnia were exposed to nominal test substance concentrations of 10, 18, 32, 58, 100 and 180 mg/L. Analytical determination of the test substance concentration was carried out at 0 and 48 h by spectrophotometry. During the test, the test substance concentrations in the analysed test media were in the range of 91.0 - 100.0 % of the nominal values. No alteration of the test substance was observed after 48 h. The average measured test substance concentrations were 9.2, 18, 32, 56, 99 and 175 mg/L. No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations.

 

Based on the above observations, the 24 and 48 h EC0 values for the test substance were established at ≥ 9.2 mg/L, and the 24 and 48 h EC100 values were established at 56 and 175 mg/L respectively. The EC50 values were calculated with the ‘Probit model’ method and found to be 47.4 mg/L at 24 h and 30.5 mg/L at 48 h.

Hence based on the above findings, the test substance is considered to be slightly toxic to Daphnia with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h exposures respectively.

Description of key information

A study was conducted to determine the acute toxicity of the test substance FAT 92368/A toDaphnia magnain a 48 h static test according to the OECD Guideline 202, Section 2, and the EU Method C.2, in GLP compliance.

Daphnia were exposed to nominal test substance concentrations of 10, 18, 32, 58, 100 and 180 mg/L. Analytical determination of the test substance concentration was carried out at 0 and 48 h by spectrophotometry. During the test, the test substance concentrations in the analysed test media were in the range of 91.0 - 100.0 % of the nominal values. No alteration of the test substance was observed after 48 h. The average measured test substance concentrations were 9.2, 18, 32, 56, 99 and 175 mg/L. No mortality was seen with blank and at 9.2 mg/L, 10 and 45 % mortality was seen at 18 and 45 mg/L, respectively, while 100 % mortality was seen with 56, 99 and 175 mg/L test concentrations.

 

Based on the above observations, the 24 and 48 h EC0values for the test substance were established at ≥ 9.2 mg/L, and the 24 and 48 h EC100values were established at 56 and 175 mg/L respectively. The EC50 values were calculated with the ‘Probit model’ method and found to be 47.4 mg/L at 24 h and 30.5 mg/L at 48 h.

Hence based on the above findings, the test substance is considered to be slightly toxic to Daphnia with EC50 values of 47.4 and 30.5 mg/L at 24 and 48 h exposures respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
30.5 mg/L

Additional information