Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of Cobaltate(3-), bis[2-[[[3-[2-[1-[[(2-chlorophenyl)amino]carbonyl]-2-(oxo-κO)propyl]diazenyl-κN1]-4-(hydroxy-κO)phenyl]sulfonyl]amino]benzoato(3-)]-, sodium (1:3) and tetrasodium bis[2-[[[3-[[1-[(2-chloroanilino)carbonyl]-2-oxopropyl]azo]-4-hydroxyphenyl]sulphonyl]amino]benzoato(3-)]cobaltate(4-)
EC number: 941-792-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- No major (the pH increased by more than 1.5 units during the test at several time points. The test is still valid, as it was shown that validity criteria are met).
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: FAT 92368/Y
Batch number: 1309001
EAS Test item code: 2014-000177
Appearance / colour: solid / orange brown
Date of analysis: 04 Oct 2013
Stability in solution: sufficient for the test purpose (at least 1h)
Expiry date: 30 Sep 2018
Storage conditions: ambient (+5 to +30 °C), dark, dry - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken at 0 hours (initial value) from fresh test solution, after 3 and 5 days from fresh and aged test solution and after 7 days from aged test solution from control and all test item concentrations. Nominal test item concentrations of 0.954, 3.05, 9.77, 31.3 and 100 mg/L and control were analysed at t=3d from fresh test solutions and at t=3d and 5d from fresh and aged test solutions.
- Vehicle:
- no
- Details on test solutions:
- The necessary amount of test item for preparing the stock solution S1 was weighed and transferred to a volumetric flask. Test medium was added up to the bench mark and the solution was homogenised by shaking. Lower concentrations of the test item were prepared by dilution of the stock solution.
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- The test organism was the aquatic plant duckweed, Lemna gibba G3 (Alismatales: Araceae). The plants used in this study were taken as aseptic clones from the cultivation of the testing facility. They were primarily cultured by Dr. Janet Slovin, Horticulture Crops Quality Laboratory, U.S. Department of Agriculture. BARC-West, Bldg. 050 HH-4, Beltsville, MD 20705, U.S.A. The plants were cultured in appropriate glass beakers under continuous illumination with OSRAM light tubes (L30/32-930 and L30/W72-965), at 6500 - 7000 lux. The temperature was 24 ± 2 °C. Before the test was started, plants were cultured under the same illumination and temperature conditions as those to be used for the toxicity test. Young, light-green plants of similar size and comprising 2 - 5 fronds were transferred onto fresh medium and cultured for approx. 3 weeks prior to testing, with two further transfers onto fresh medium before initiating the test.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Hardness:
- none
- Test temperature:
- 22.0 – 25.0 °C
- pH:
- 7.47 – 7.63
- Dissolved oxygen:
- none
- Salinity:
- none
- Conductivity:
- none
- Nominal and measured concentrations:
- 0.954, 3.05, 9.77, 31.3 and 100 mg/L
- Details on test conditions:
- Light intensity (mean): ~ 6800 lux
Light tubes: OSRAM light tubes (L 30 W77 and L 30 W25)
Exposure to light: continuously
Test vessels: 250 mL glass beakers filled with 150 mL
Number of fronds: 12 per test vessel - Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.272 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of frond number
- Remarks on result:
- other: 0.000545-1.32
- Duration:
- 7 d
- Dose descriptor:
- EC20
- Effect conc.:
- 0.856 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of frond number
- Remarks on result:
- other: 0.0116-3.00
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 7.69 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of frond number
- Remarks on result:
- other: 1.83-32.0
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.534 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of frond number
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.534 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of frond number
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.837 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of frond number
- Remarks on result:
- other: 0.0000125-4.21
- Duration:
- 7 d
- Dose descriptor:
- EC20
- Effect conc.:
- 4.05 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of frond number
- Remarks on result:
- other: 0.0165-14.9
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 96 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of frond number
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.534 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of frond number
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of frond number
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.428 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of dry weight
- Remarks on result:
- other: 0.0120-1.52
- Duration:
- 7 d
- Dose descriptor:
- EC20
- Effect conc.:
- 1.24 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of dry weight
- Remarks on result:
- other: 0.102-3.40
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 9.38 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of dry weight
- Remarks on result:
- other: 3.41-28.2
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.534 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of dry weight
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield of dry weight
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 1.33 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of dry weight
- Remarks on result:
- other: 0.0101-4.84
- Duration:
- 7 d
- Dose descriptor:
- EC20
- Effect conc.:
- 5.45 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of dry weight
- Remarks on result:
- other: 0.411-15.3
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 96 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of dry weight
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.534 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of dry weight
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate of dry weight
- Reported statistics and error estimates:
- The statistical evaluation for day 7 was performed for yield of frond numbers, growth rate of frond numbers, growth rate dry weight and yield of dry weight. A test for normality of the data was performed by calculating the Shapiro-Wilk’s statistic, a test for homogeneity of the data was performed according to Levene. The NOEC and LOEC were determined by using a multiple comparison method (Dunnett`s t-test and Jonckheere-Terpstra). EC10 and EC20 values were determined by probit analysis following normal or logistic procedure. The EC50 value for yield of frond number and yield of dry weight was determined by probit analysis following normal procedure. The calculation of EC50 values for growth rate of frond numbers and growth rate of dry weight was not indicated since inhibition was below 50 %. The evaluation of data was performed by SAS® (2002-2010).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The nominal EC50-value for yield of frond numbers was determined to be 10.4 mg/L, the nominal EC50-value for yield of dry weight was determined to be 12.4 mg/L. The nominal EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >100 mg/L. The actual EC50-value for yield of frond numbers was determined to be 7.69 mg/L, the actual EC50-value for yield of dry weight was determined to be 9.38 mg/L. The actual EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >96.0 mg/L.
- Executive summary:
FAT 92368/Y was tested for toxicity to plants other than algae. This test was conducted according to OECD TG 221. The initial mean measured concentration of FAT 92368/Y in the test item solutions was 88 % of nominal. The mean measured content of FAT 92368/Y in aged test item solutions was 62 %. Therefore, toxicological endpoints were evaluated using nominal concentrations of the test item and actual concentrations based on the geometric mean concentration of each test item concentration level. Significant inhibitory effects were determined for yield of frond numbers at all concentration levels. For growth rate of frond numbers, yield of dry weight and growth rate of dry weight significant inhibitory effects were observed at 3.05, 9.77, 31.3 and 100 mg/L (nominal). The LOEC for yield of frond numbers was determined to be 0.954 mg/L (nominal), the LOEC for growth rate of frond numbers, yield of dry weight and growth rate of dry weight was at 3.05 mg/L (nominal). The nominal EC10-value for yield of frond numbers was determined to be 0.513 mg/L, the nominal EC10-value for yield of dry weight was determined to be 0.771 mg/L. The nominal EC10-value for growth rate of frond numbers was determined to be 1.41 mg/L and the nominal EC10-value for growth rate of dry weight was determined to be 2.14 mg/L. The actual EC10-value for yield of frond numbers was determined to be 0.272 mg/L, the actual EC10-value for yield of dry weight was determined to be 0.428 mg/L. The actual EC10-value for growth rate of frond numbers was determined to be 0.837 mg/L and the actual EC10-value for growth rate of dry weight was determined to be 1.33 mg/L. The nominal EC20-value for yield of frond numbers was determined to be 1.44 mg/L, the nominal EC20-value for yield of dry weight was determined to be 2.00 mg/L. The nominal EC20-value for growth rate of frond numbers was determined to be 5.82 mg/L and the nominal EC20-value for growth rate of dry weight was determined to be 7.61 mg/L. The actual EC20-value for yield of frond numbers was determined to be 0.856 mg/L, the actual EC20-value for yield of dry weight was determined to be 1.24 mg/L. The actual EC20-value for growth rate of frond numbers was determined to be 4.05 mg/L and the actual EC20-value for growth rate of dry weight was determined to be 5.45 mg/L. The nominal EC50-value for yield of frond numbers was determined to be 10.4 mg/L, the nominal EC50-value for yield of dry weight was determined to be 12.4 mg/L. The nominal EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >100 mg/L. The actual EC50-value for yield of frond numbers was determined to be 7.69 mg/L, the actual EC50-value for yield of dry weight was determined to be 9.38 mg/L. The actual EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >96.0 mg/L. The test is valid as the doubling time of control frond numbers was calculated to be 47.7 h, corresponding to 1.987 days at day 7. This is less than the maximum allowable doubling time set in OECD guideline 221 (60 h, 2.5 days).
Reference
|
Test item [mg/L] nominal |
|||||||
Endpoint |
EC10 |
95 % confidence limit |
EC20 |
95 % confidence limit |
EC50 |
95 % confidence limit |
NOEC |
LOEC |
Yield of frond numbers |
0.5131) |
0.00208-2.10 |
1.441) |
0.0318-4.43 |
10.41) |
2.88 – 37.0 |
<0.9542) |
0.9542) |
Growth rate of frond numbers |
1.414) |
0.0000861-6.00 |
5.824) |
0.0471-18.6 |
>100 |
- |
0.9542) |
3.052) |
Yield of dry weight |
0.7711) |
0.0313-2.41 |
2.001) |
0.213-4.96 |
12.41) |
5.00 – 33.2 |
0.9543) |
3.053) |
Growth rate of dry weight |
2.144) |
0.0283-6.81 |
7.614) |
0.766-19.2 |
>100 |
- |
0.9543) |
3.053) |
1) Probit analysis following the normal distribution
2) following Dunnetts-t-test
3) following Jonckheere-Terpstra
4) Probit analysis following the logistic distribution
*based on geometric mean of each concentration level
- not applicable
Validity of test :The test is valid as the doubling time of control frond numbers was calculated to be 47.7 h, corresponding to 1.987 days at day 7. This is less than the maximum allowable doubling time set in OECD guideline 221 (60 h, 2.5 days).
Description of key information
FAT 92368/Y was tested for toxicity to plants other than algae. This test was conducted according to OECD TG 221. The initial mean measured concentration of FAT 92368/Y in the test item solutions was 88 % of nominal. The mean measured content of FAT 92368/Y in aged test item solutions was 62 %. Therefore, toxicological endpoints were evaluated using nominal concentrations of the test item and actual concentrations based on the geometric mean concentration of each test item concentration level.
Toxicological endpoints for FAT 92368/Y:
Significant inhibitory effects were determined for yield of frond numbers at all concentration levels. For growth rate of frond numbers, yield of dry weight and growth rate of dry weight significant inhibitory effects were observed at 3.05, 9.77, 31.3 and 100 mg/L (nominal). The LOEC for yield of frond numbers was determined to be 0.954 mg/L (nominal), the LOEC for growth rate of frond numbers, yield of dry weight and growth rate of dry weight was at 3.05 mg/L (nominal). The nominal EC10-value for yield of frond numbers was determined to be 0.513 mg/L, the nominal EC10-value for yield of dry weight was determined to be 0.771 mg/L. The nominal EC10-value for growth rate of frond numbers was determined to be 1.41 mg/L and the nominal EC10-value for growth rate of dry weight was determined to be 2.14 mg/L.
The actual EC10-value for yield of frond numbers was determined to be 0.272 mg/L, the actual EC10-value for yield of dry weight was determined to be 0.428 mg/L. The actual EC10-value for growth rate of frond numbers was determined to be 0.837 mg/L and the actual EC10-value for growth rate of dry weight was determined to be 1.33 mg/L.
The nominal EC20-value for yield of frond numbers was determined to be 1.44 mg/L, the nominal EC20-value for yield of dry weight was determined to be 2.00 mg/L. The nominal EC20-value for growth rate of frond numbers was determined to be 5.82 mg/L and the nominal EC20-value for growth rate of dry weight was determined to be 7.61 mg/L.
The actual EC20-value for yield of frond numbers was determined to be 0.856 mg/L, the actual EC20-value for yield of dry weight was determined to be 1.24 mg/L. The actual EC20-value for growth rate of frond numbers was determined to be 4.05 mg/L and the actual EC20-value for growth rate of dry weight was determined to be 5.45 mg/L.
The nominal EC50-value for yield of frond numbers was determined to be 10.4 mg/L, the nominal EC50-value for yield of dry weight was determined to be 12.4 mg/L. The nominal EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >100 mg/L.
The actual EC50-value for yield of frond numbers was determined to be 7.69 mg/L, the actual EC50-value for yield of dry weight was determined to be 9.38 mg/L. The actual EC50-value for growth rate of frond numbers and growth rate of dry weight was determined to be >96.0 mg/L.
The test is valid as the doubling time of control frond numbers was calculated to be 47.7 h, corresponding to 1.987 days at day 7. This is less than the maximum allowable doubling time set in OECD guideline 221 (60 h, 2.5 days).
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 96 mg/L
- EC10 or NOEC for freshwater plants:
- 0.837 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
