1-(3-chlorophenyl)-4-(3-chloropropyl)piperazinium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material

GLP compliance:

no

Analytical monitoring:

no

Remarks:

stable under recommended conditions

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 82 mg, 114.8 mg, 160.7 mg, 225 mg, 315.01 mg & 1 gm of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, respectively

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium
- Length at study initiation (length definition, mean, range and SD):average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.115 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

52.2 mg of CaCO3

Test temperature:

29°C

pH:

7.3

Dissolved oxygen:

7.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12 inch each.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod:12 hours light and 12-hour darkness

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 11.48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: other details not available

Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
8.2	8	-	-	-	-
11.48	8	-	-	-	-
16.072	8	8	-	-	-
22.50	8	8	-	-	-
31.50	8	8	-	-	-
100	8	8	-	-	-

Visible Symptoms: Fish were swimming slowly and settled down like they are dead

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
8.2	8	NS8	NS8	NS8	NS8
11.48	8	NS8	NS8	NS8	NS8
16.072	8	NS0	NS0	NS0	NS0
22.50	8	NS0	NS0	NS0	NS0
31.50	8	NS0	NS0	NS0	NS0
100	8	NS0	NS0	NS0	NS0

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

Nominal conc. (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.7	7.6	7.2	7.3
8.2	7.8	6.0	7.7	7.9
11.48	6.1	7.9	7.8	7.9

Nominal conc. (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	6.04	7.6	5.38	5.52
8.2	5.45	5.60	5.18	5.34
11.48	5.48	5.99	5.14	5.16

Test temperature:

Nominal conc. (mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	28.8	30.2	31	31.5
8.2	29.8	28.4	30.5	29.9
11.48	30.1	28.6	30.2	30.4

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] of test chemical on Zebra Fish Danio rerio was determined to be >11.48 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical on Zebra fish. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 82 mg, 114.8 mg, 160.7 mg, 225 mg, 315.01 mg & 1 gm of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, respectively . Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fish. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was determined to be 11.48 mg/L..

After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be >11.48 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be cclassified as aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical on Zebra fish. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, and Zebra FishDanio reriowere exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 82 mg, 114.8 mg, 160.7 mg, 225 mg, 315.01 mg & 1 gm of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, respectively . Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fish. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was determined to be 11.48 mg/L..

After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be 11.48 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be cclassified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

11.48 mg/L

Additional information

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical on Zebra fish. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, and Zebra FishDanio reriowere exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 82 mg, 114.8 mg, 160.7 mg, 225 mg, 315.01 mg & 1 gm of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L, respectively . Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fish. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The nominal concentrations selected for the experiment were 8.2 mg/L, 11.48 mg/L, 16.072 mg/L, 22.500 mg/L, 31.501 mg/L & 100 mg/L and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was determined to be 11.48 mg/L..

After 96 hours of exposure to test chemical to various nominal test concentrations, LC50 was determine to be 11.48 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be cclassified as aquatic chronic 3 as per the CLP classification criteria.