1-(3-chlorophenyl)-4-(3-chloropropyl)piperazinium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: estimated by calculation

Justification for type of information:

estimated by calculation

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT).  It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA).  It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured.  Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow).  Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3.079 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

not specified

Conclusions:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 3.079 mg/L.

Executive summary:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 3.079 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and can be classified aquatic chronic 2 .

Description of key information

Short-term toxicity to aquatic invertebrates:

The Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 3.079 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and can be classified aquatic chronic 2 .

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.079 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

The toxicity of test material to aquatic invertebrate based on prediction along with data from peer reviewed journal and handbook for structurally and functionally similar read across substance.

In the predicted data , the Lethal concentration (LC50) value of test material in aquatic invertebrate (Daphnia Magna) in a 48 hr study based on mortality effect was estimated to be 3.079 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance , it is concluded that test material exhibit short term toxicity aquatic invertebrate (Daphnia Magna) and can be classified aquatic chronic 2 .

The above prediction was further supported by data for structurally similar read across substance ,the effect of test material on aquatic invertebrate Daphnia magna was observed for 48 h in accordance with OECD 2002 guideline.Monoclonal cultures of Daphnia magna were maintained in the laboratory for several generations in synthetic hard water medium –ASTM supplemented with an organic additive (suspension extracted from Ascophyllum nodosum). The cultures were maintained under a temperature of 20 ±2 C and a photoperiod of 16L:8D and were regularly renewed using neonates from 3rd to 5th broods. The organisms were fed thrice weekly, with a ration of 3.0x105 cells /ml/day of the algae Pseudokirchneriella subcapitata.

The acute assays were conducted in glass tubes containing 10mL of test solution and 10mL of clean ASTM medium (negative control).

Twenty animals (524 h old) divided into four groups of five animals per treatment concentration were used. The nominal concentration was in the range of 0.50–3.14 mg/l.After 48-h tests the immobile/dead organisms were observed, counted and registered. The EC50 value of test material observed after 48 h was 1.81 mg/l and the 95% confidence interval was 1.1 - 4.5 mg/l. Based on the effect concentration it can be considered that test material has toxic effects in aquatic invertebrate and can be classified as aquatic chronic- 2 as per CLP criteria.

The above data was further supported by data from handbook for functionally similar read across substance, short term toxicity of test material was evaluated for 48 h the effect concentation EC50 was observed to be 2.4 mg/l . Hence based on the above effect concentration it can be considered that test material was toxic to aquatic invertebrate, and can be classified as aquatic chronic 2.