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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
1 000 mg/kg soil dw

Additional information

A study by Witte, B. 2010 was carried out to determine the toxicity of fatty acids, C18 reaction products with 1 -piperazineethanamine on the reproduction and growth of earthwoms.

Eisenia fetida were exposed to Fatty acids, C16-18, reaction products with 1-piperazineethanamine at a range of concentrations composing of 0, 62.5, 125, 250, 500 and 1000 mg of Fatty acids, C16-18, reaction products with 1-piperazineethanamine/kg soil dry weight corresponding to 58.8, 117.5, 235, 470 and 940 mg a.i./kg soil dry weight. The adult worms were between 11 and 12 months old an were exposed to the test substance for 56 days. The substrate used was artifical soil. There were 6 treatment groups with 4 replicates for the test item treatments and 8 replicates for the control with 10 worms each.

The worms were assessed for adult worm mortality, behavioural effects and biomass development after 28 days of exposure. The reproduction rate was assessed after an additional 28 days.

All study validity criteria were met. No mortality was observed in any treatment group. The body weight change of the earthworms after 4 weeks exposure for Fatty acids, C16-18, reaction products with 1-piperazineethanamine was not statistically significant different compared to the control up to and including the highest test concentration of 1000 mg test item/kg soil dry weight (Dunnett's test,α = 0.05).

The reproduction rates after 8 weeks exposure to Fatty acids, C16-18, reaction products with 1-piperazineethanamine were not significantly different compared to the control up to and including the highest test concentration of 1000 mg test item/kg soil ( Dunnett's t-test,α = 0.05). No behavioural abnormalities were observed in any of the treatment groups.