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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December 17th, 1993 - March 3rd, 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed according to Japan Guideline and with GLP compliance, but the raw data are presented in the report).
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
other: Bioconcentration Test of Chemical substances in fish and shells, as laid down in "Testing methods relative to new chemical substances" (Kan-ho-gyo n°5, Yaku-hatsu n°615, 49 kikyoku n°392, July 1974)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: 9221301
- Expiration date of the lot/batch: not specified

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in a dark and cool place
- Stability under test conditions: verified before the initiation of exposure
Radiolabelling:
no
Vehicle:
no
Details on preparation of test solutions, spiked fish food or sediment:
Taking into account the preliminary value of the 48h-LC50 and the analysis of the substance to be tested, the concentration of the test substance was fixed at:
- first section 0.2 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L (the test substance was dissolved in deionized water, and the mother solution concentration was adjusted to 40 mg/L)
- second section 0.02 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L (the test substance was dissolved in deionized water, and the mother solution concentration was adjusted to 4 mg/L)
At the same time, a reference section was set up as a blank.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Sugishima fish breeding center
- Date of reception of the test fish : 19th october, 1993
- Length at study initiation: average 9.4 cm
- Weight at study initiation: average 22.4 g
- Lipid content at test initiation: average 3.5%
- Breeding conditions: At the time when the fish were received, abnormal specimen were eliminated through visual inspection, and the fish were immersed into medicated water in the reception tank. They were then raised for 7 days under running water
- Feeding during test
- Food type: feed pellets for carp
- Amount/frequency: An amount corresponding to about 2% of the test fish weight was fed in 2 portions each day. However, feeding was stopped the day before the test fish were sampled.

ACCLIMATION
- Acclimation conditions: After breeding and immersion into a medicated tank to eliminate parasites, the wish were transferred to the acclimation tank, they were immersed once more in medicated water, and acclimation was carried out. During that time abnormal specimen were eliminated, and the rest were raised for 25 days under running water at a water temperature of 25.2°C. Then they were transferred to the testing water tank, and after immersion into medicated water they were raised for 27 days under running water at the same temperature.
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Test temperature:
25±2°C
Dissolved oxygen:
- First concentration section: 6.5-7.8 mg/L
- Second concentration section: 6.6-7.8 mg/L
- Reference section: 7.2-7.9 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass water tank
- Material, size, headspace, fill volume: 100 l
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): 579 l/day were supplied to the water tank in the following proportion: mother solution 2 ml/min and test water 400 ml/min
- No. of organisms per vessel: 11 (at start of exposure) in the first and the second sections / 5 (at start of exposure) in the reference section
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control / vehicle control (replicates): not specified
- Biomass loading rate: not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: underground water raised on the grounds of Kurumei Research Center
- Intervals of water quality measurement: periodical analysis (once every 6 months) of total hardness, evaporation residue, chemical oxygen demand, free chlorine, ammonia nitrogen as well as of noxious substances such as organic phosphorus, cyanide ion and heavy metals

OTHER TEST CONDITIONS
- Photoperiod: not specified

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: 8.78, 13.2, 19.8, 29.6, 44.4, 66.7, 100 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide:L
Nominal and measured concentrations:
The concentration of the test substance was fixed at:
- first section 0.2 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L
- second section 0.02 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L
Analysis of the test substance in test water was carried out twice a week both in concentration section 1 and in concentration section 2 during the exposure period, 16 times in total (one sample each time). Additionally, analysis of the test substance in test fish was carried out 2 - 4 - 6 and 8 weeks after the initiation of exposure both in concentration section 1 and in concentration section 2, 4 times in total. The analysis sample consisted of 2 fish each time. Analysis in the reference section was carried out before the initiation of exposure and after the terminaison of exposure, the analysis sample consisting of 2 fish each time.
Quantitative analysis were performed by HPLC.
Reference substance (positive control):
no
Conc. / dose:
0.2 mg/L
Type:
BCF
Value:
< 0.5 dimensionless
Basis:
not specified
Remarks on result:
other: based on the analytical monitoring of N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide
Conc. / dose:
2 mg/L
Type:
BCF
Value:
< 5.3 dimensionless
Basis:
not specified
Remarks on result:
other: based on the analytical monitoring of N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide
Details on kinetic parameters:
no data
Metabolites:
no data
Details on results:
- Mortality of test organisms: visual inspection did not show any defect on test fish
- Behavioural abnormalities: visual inspection did not show any defect on test fish
- Observations on feeding behavior: not specified
- Observations on body length and weight: not specified
- Reproduction during test period: not specified
- Organ specific bioaccumulation: not specified
- Bound residues forming a plateau: not specified
- Mortality and/or behavioural abnormalities of control: not specified
- Loss of test substance during test period: not specified
- Non-eliminated residues (NER) at the end of elimination phase: not specified

The concentration of the test substance in test water is shown in Table 1. The average concentration of the test substance in test water was maintained at more than 80% of the set value.

Table 1 - Concentration of the test substance in test water (average of the values obtained from the initiation of exposure)

   2 weeks 4 weeks  6 weeks   8 weeks
 Concentration section 1 (mg/L)  0.162  0.168  0.172  0.174
 Concentration section 2 (mg/L)  0.017  0.0178  0.0180  0.0181

 

The bioconcentration factor is shown in Table 2. The bioconcentration factor was found to be less than 0.5 in concentration section 1, and less than 5.3 in concentration section 2.

Table 2 - Concentration of the test substance in test water (average of the values obtained from the initiation of exposure)

   2 weeks 4 weeks  6 weeks   8 weeks
 Concentration section 1 (mg/L)  <0.5  <0.5  <0.5  <0.5
 Concentration section 2 (mg/L)  <5.3  <5.3  <5.3  <5.3

 

Validity criteria fulfilled:
not specified
Conclusions:
A 8-week bioaccumulation study was performed under flow-through conditions with Cyprinus carpio according to OECD Test Guideline 305C and to GLP. Fishes were exposed to two test concentrations, 0.2 and 0.002 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L. The BCF obtained at these concentrations were <0.5 and <5.3, respectively.
Executive summary:

A 8-week bioaccumulation study was performed under flow-through conditions with Cyprinus carpio according to OECD Test Guideline 305C and to GLP. Fishes were exposed to two test concentrations, 0.2 and 0.002 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L. The BCF obtained at these concentrations were <0.5 and <5.3, respectively.

Description of key information

 A 8-week bioaccumulation study was performed under flow-through conditions with Cyprinus carpio according to OECD Test Guideline 305C and to GLP. Fishes were exposed to two test concentrations, 0.2 and 0.002 mg N-[2-(2-oxo-1-imidazolidinyl)ethyl] methacrylamide/L. The BCF obtained at these concentrations were <0.5 and <5.3, respectively. 

Key value for chemical safety assessment

Additional information