Registration Dossier
Registration Dossier
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EC number: 609-664-1 | CAS number: 393-85-1
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1993-11-03-1993-11-03
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-Fluoro-2-nitrobenzoic acid methyl ester
- EC Number:
- 609-664-1
- Cas Number:
- 393-85-1
- Molecular formula:
- C8H6FNO4
- IUPAC Name:
- 5-Fluoro-2-nitrobenzoic acid methyl ester
- Details on test material:
- Purity: 96+/- 0.2 %
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- The inoculum ( activated sludge) was not acclimatised before the experiment started.
Test conditions
- Test temperature:
- 20+/- 2 °C
- pH:
- 7.2 +/- 0.2
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 729.9 mg/L
- Nominal / measured:
- nominal
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 10 - 100 mg/L
- Nominal / measured:
- nominal
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 80 mg/L
- Nominal / measured:
- nominal
- Results with reference substance (positive control):
- 3,5-Dichlorophenol at a concentration of 5, 20 and 30 mg/l
Applicant's summary and conclusion
- Conclusions:
- Based on this study it can be stated that the EC50 is 729.9 mg/l. 5-Fluoro-2-nitrobenzoic acid methyl ester is not harmful to the activated sludge.
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