Alkali salt of substituted aryl sulfonyl triazinyl amino hydroxy sulfonyl aryl diazo naphthalene sulfonate

Partition coefficient

Administrative data

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 May 2011 to 12 May 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to EU & OECD test guidance in compliance with GLP and reported with a valid GLP certificate.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

shake-flask method to: flask method

Partition coefficient type:

octanol-water

Test material

Test material information

Constituent 1

Details on test material:

Name: Reactive Red F08-0146

Study design

Analytical method:

high-performance liquid chromatography

Results and discussion

Partition coefficient

Type:

log Pow

Partition coefficient:

< -4.4

Remarks on result:

other: pH and temp not specified

Details on results:

Analysis of the two-phase system
Measured concentrations in both phases of the test vessels are provided in Table 1 below. Reactive Red F08-0146 was not detected in the n-octanol phase; therefore less than the LOQ given in Table 1 below. The calculated Pow values are summarised in Table 2 below.

Any other information on results incl. tables

Table 1: Measured concentrations

Ratio of solvents (n-Octanol: Water)	Measured concentration of Reactive Red F08-0146in n-octanol phase (µg/ml)		Measured concentration of Reactive Red F08-0146in water phase (µg/ml)	pH
1:1	< 0.25* < 0.25		7250 7331	4.69
	< 0.25 < 0.25		7308 7280	4.68
	< 0.25 < 0.25		7226 7357	4.62
Mean (C)	< 0.25		7292	4.66
RSD%	-		1	-
Meas. test item (M)	< 6.25 µg		182 mg	-
Total quantity of test item (Mo+Mw) in the two-phase system:		182 mg
Recovery:		98 %
2:1	< 0.25* < 0.25		11269 11372	4.50
	< 0.25 < 0.25		10965 11115	4.50
	< 0.25 < 0.25		11194 11215	4.47
Mean (C)	< 0.25		11188	4.49
RSD%	-		1	-
Meas. test item (M)	< 8.4 µg		185 mg	-
Total quantity of test item (Mo+Mw) in the two-phase system:		185 mg
Recovery:		99 %

*   Corresponds to the LOQ after the extraction

Table 1.: Measured concentrations (continued)

Ratio of solvents (n-Octanol: Water)	Measured concentration of Reactive Red F08-0146in n-octanol phase (µg/ml)		Measured concentration of Reactive Red F08-0146in water phase (µg/ml)	pH
1:2	< 0.25* < 0.25		5510 5563	4.68
	< 0.25 < 0.25		5615 5627	4.67
	< 0.25 < 0.25		5469 5519	4.67
Mean (C)	< 0.25		5550	4.67
RSD%	-		1	-
Meas. test item (M)	< 4.1 µg		186 mg	-
Total quantity of test item (Mo+Mw) in the two-phase system:		186 mg
Recovery:		100 %

*   Corresponds to the LOQ after the extraction

 

Table 2.: Calculated partition coefficient (P_ow) and log P_ow

Ratio of solvents (n-Octanol: Water)	Partition coefficient	Log Partition coefficient
	Pow	Log Pow
1:1	< 0.000034	< -4.5
2:1	< 0.000022	< -4.7
1:2	< 0.000045	< -4.4

Applicant's summary and conclusion

Conclusions:

In conclusion, the partition coefficient (Pow) of Reactive Red F08-0146 in a n octanol - water two-phase system was determined to be less than 4.5 * 10-5 using shake flask method. The log Pow is < -4.4.

Executive summary:

This study has been performed in accordance with the study plan, EU and OECD test guidance and the Principles of Good Laboratory Practice .

Summary of the results:

Partition coefficient (P_ow) ofReactive Red F08-0146was determined using the shake-flask method:

P_ow:< 4.5 *10^-5

log P_ow: < -4.4