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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (non GLP, no analytical concentration verification)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-nitrophenol
EC Number:
201-857-5
EC Name:
2-nitrophenol
Cas Number:
88-75-5
Molecular formula:
C6H5NO3
IUPAC Name:
2-nitrophenol
Details on test material:
- Name of test material (as cited in study report): o-Nitrophenol
- Analytical purity: no data
Radiolabelling:
not specified

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk

Test conditions

Test temperature:
25 ± 2 °C
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volume = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 mL/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20

TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week
Nominal and measured concentrations:
1 mg/L and 0.1 mg/L

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
< 5
Remarks on result:
other: Conc.in environment / dose:1 mg/L
Type:
BCF
Value:
< 22
Remarks on result:
other: Conc.in environment / dose:0.1 mg/l

Applicant's summary and conclusion