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The lowest concentration without toxic effects after 48 h exposure to fish is 25 mg/L.

The EC50,48h of dimethylmaleate for Daphnia magna is 6.51 mg/L. The NOEC and LOEC were determined to 2 and 4 mg/L, respectively.

The EC50,72h,growth of dimethylmaleate for algae is 13.34 mg/L.

An EC10,3h of 100 mg/L was derived for the inhibition of the respiration of microorganisms from activated sludge.