3,7,11,15-tetramethylhexadec-1-en-3-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

EC Number:

208-008-8

EC Name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

Cas Number:

505-32-8

Molecular formula:

C20H40O

IUPAC Name:

3,7,11,15-tetramethylhexadec-1-en-3-ol

Details on test material:

- Name of test material (as cited in study report): 3,7,11,15-Tetramethyl-1-hexadecen-3-ol (Isophytol)
- Analytical purity: 97.6 %
Water solubility: insoluble

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fish farm, Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range 5.7 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.9 g (range 1.5 - 4.4 g)
- Corpulence factor of the test fish batch: 0.95
- Feeding during test: 1 day before and during exposure.
- Housing: Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system; Water temperature: 20 - 21 °C Duration of housing: about 2 months Mortality during the last 2 weeks of housing: about 2.3 %. Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum.
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

20 (+/- 1) °C

pH:

7.2 - 7.8

Dissolved oxygen:

8.3 - 9.0 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0, 5000, and 10000

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 5.0 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/l CaCl2 * 2 H2O; 123.3 mg/l MgSO4 + 7 H2O, 63.0 mg/l NaHCO3 5.5 mg/l KCl
- Ration Na/K ions: 10 : 1
- Acid capacity: 0.8 mmol/l
- Ca/mg ratio: 4 : 1



OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

48 h-LC50 of the positive control Chloroacetamide: about 32 mg/l (corresponds to normal sensitivity).

Reported statistics and error estimates:

NOEC and LC50 were determined directly from the raw data.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to isophytol, mortalities were as follows:

Nominal test Cumulative mortality (%) after x hours
concentration 1 /4 /24/48/72/96/
(mg/l)
Control 0 /0 /0 /0 /0 /0 /
5000 0 /0 /0 /0 /0 /0 /
10000 0 /0 /0 /0 /0 /0 /


Symptoms of intoxication:

Nominal test Symptoms of intoxication after x hours
concentration /1 /4 /24/48/72/96/
(mg/l)
Control /- /- /- /- /- /- /
5000 /- /- /- /- /- /- /
10000 /- /- /- /- /- /- /


Definitions of Symptoms:
-: No symptoms

All results refer to nominal test concentrations of isophytol.
No analytical dose-verification of the test item was carried out.