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Acute Toxicity: inhalation

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acute toxicity: inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: secondary literature

Data source

Reference Type:
secondary source

Materials and methods

Principles of method if other than guideline:
Study to determine the median lethal concentration (LC50), PFOS dust was administered in air to Sprague-Dawley rats, 5/sex/group, levels of 1.89 to 45.97 mg/l PFOS to eight test groups. A Wright dust-feed mechanism with dry air at a flow rate of 12 to 16 liters per minute was used to administer the PFOS dust. Rats were exposed for 1 hour. Rats were observed for abnormal clinical signs, the body weight was recorded and a necropsy was conducted on animals which died spontanously and after termination of the study after 14 days
GLP compliance:
Test type:
standard acute method

Test material

Constituent 1
Reference substance name:
Potassium heptadecafluorooctane-1-sulphonate
EC Number:
EC Name:
Potassium heptadecafluorooctane-1-sulphonate
Cas Number:
potassium 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluorooctane-1-sulfonate
Details on test material:
Identity: Potassium perfluorooctylsulfonate, CAS No.: 2795-39-3
Remarks: Dust, PFOS, Purity not specified.

Test animals


Administration / exposure

Route of administration:
inhalation: dust
Type of inhalation exposure:
whole body
Duration of exposure:
1 h
0.0, 1.89, 2.86, 4.88, 6.49, 7.05, 13.9, 24.09 or 45.97 mg/l
No. of animals per sex per dose:
Control animals:

Results and discussion

Effect levels
Dose descriptor:
Effect level:
5.2 mg/L air
Based on:
test mat.
Exp. duration:
1 h

Any other information on results incl. tables

The highest dose group, 45.97 mg/l, was not used in the LC50 calculations and terminated on Day 2. At that point, only 5 animals survived and blood samples were taken at termination. The 13.9 mg/l group was also terminated early (Day 1) because of a mechanical problem during exposure. These animals were also not used in the LC50 determination.

In the 24.09 mg/l exposure group, all animals died by Day 6. At 7.05 and 6.49 mg/l there was 80% mortality. At 4.88, 2.86, and 1.89 mg/l there was 20%, 10%, and 0% mortality, respectively. The rats in all these groups showed signs of toxicity including emaciation, red material around the nose or other nasal discharge, yellow material around the anogenital region, dry rales or other breathing disturbances, and general poor condition. Abnormal in-life observations were reported to be less frequent in the lower

exposure groups.

At necropsy, the most common abnormality was discoloration of the liver and lung. Discoloration of the lung was also observed in control rats and therefore may not be treatment related. Therefore, the most significant treatment related abnormality was varying degrees of discoloration of the liver. Among animals that died prematurely, decreased body weight, discoloration of the lung, and discoloration and distention of the small intestine were also observed.

Applicant's summary and conclusion

Interpretation of results:
Migrated information
Executive summary:

Rats were exposed for 1 hour to PFOS dust at concentrations of 1.89, 2.86, 4.88, 6.49, 7.05, 13.9, 24.09, 45.97 mg/l.The rats were observed for abnormal signs prior to exposure, at 15-minute intervals during the 1-hour exposure, at removal from the exposure chamber, hourly for four hours after exposure, and daily thereafter for 14 days. Individual bodyweights were recorded on Day 0 (prior to exposure), Day 1, Day 2, Day 4, Day 7, and Day 14. All animals dying spontaneously were necropsied as soon as possible after death.

LC50 = 5.2 (4.4 – 6.4) mg/l.