Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

One key study is available for each of the following endpoints:




The acute toxicity of 4-methyl-3-thiosemicarbazide was evaluated with Danio rerio: LC50-96 h was estimated using measured concentrations as being equal to 20 mg/L.


Aquatic invertebrates


The acute toxicity of 4-methylthiosemicarbazide was evaluated with Daphnia magna in a study performed in accordance with OECD testing guideline 202 (part I) and GLP requirements. EC50-48 h was estimated using measured concentrations (geometric mean) as being equal to 3.1 mg/L.




The determination of the growth inhibition of the freshwater algae Pseudokirchneriella subcapitata exposed to the test item 4-methyl-3-thiosemicarbazide for a duration of 72 hours was assessed according to the OECD Guideline 201. ErC50-72h was determined as 30 mg/L




No inhibition of respiration of activated sludge was observed up to 100 mg/L the highest tested concentration in an OECD 209 compliant study.