3,4-xylidine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Data source

Reference

Reference Type:

review article or handbook

Title:

Results of the Harmful Effects of Water Pollutants to Daphnia magna in the 21 Day Reproduction Test

Author:

Kühn, R.; Pattard, M.; Pernak, K. D.; Winter, A.

Year:

1989

Bibliographic source:

Water Research, 23 (4), 1989, S. 501-510

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Investigations were carried out in order to determine the “no observed effect concentration” (NOEC) of 73 environmentally relevant substances in the 21 d Daphniareproductiontest. The test was conducted in line with the provisional procedure proposed by the Federal Environmental Agency (Umweltbundesamt) (as of 1 January 1984). Daphniamagna Straus was used as the test organism

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3,4-xylidine

EC Number:

202-437-4

EC Name:

3,4-xylidine

Cas Number:

95-64-7

Molecular formula:

C8H11N

IUPAC Name:

3,4-dimethylaniline

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

21 d

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.016 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

Effect : It effects Reproduction

Applicant's summary and conclusion

Conclusions:

Endpoints : NOEC
Organism : Daphnia magna
Dur Normalized : 21 d
normalised : 0.016
Unit normalised : mg/l
Test Medium : Freshwater
Effect : Reproduction

Executive summary:

Investigations were carried out in order to determine the “no observed effect concentration” (NOEC) of 73 environmentally relevant substances in the 21d Daphnia reproduction test. The test was conducted in line with the provisional procedure proposed by the Federal Environmental Agency (Umweltbundesamt) (as of 1 January 1984). Daphnia magnaStraus was used as the test organism. Evaluation parameters for fixing the NOEC were the mortality of the parent animals, the reproduction rate and the appearance of the first offspring during the test period. The concentrations of substances in thetestpreparations were chemically quantified. The NOEC values obtained for ethyl parathion, bis(tri-n-butyltin)oxide and the active ion Cd (II) were in the concentration range 1 ng 1−1−1 μg 1−1, for I3 tested substances in the range 1 μg 1−1−< 0.1 mg 1−1and for 23 substances in the range 0.1 −< 1 mg 1−1. In further evaluation of theresults, it could be calculated that the substance concentration of the NOEC for three substances was more than 3 logs, in the case of 13 substances more than 2 logs, for 10 substances 50–90 times, for 24 substances 10–40 times and for 14 substances 5–9 times lower than the 24 h EC50of the corresponding substance.