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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given (comparable to guidelines/standards)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976

Materials and methods

Principles of method if other than guideline:
Method by US-EPA 1976 (EPA-660/3-76-097)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
3-nitrotoluene
Details on test material:
- Name of test material (as cited in study report): m-Nitrotoluene
- Analytical purity: "reagent grade"

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Juveniles 4 to 8 weeks old, not fed during the test.
Total lenght: 1.1-3.1 cm
20 individuals tested at each concentration
Fish were acclimated in flowing water for at least 48 h before the test.
During acclimation the fishes were fed.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
18-22 °C
pH:
< 5.9
Dissolved oxygen:
< 4.0 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
1 h
Dose descriptor:
LC50
Effect conc.:
43 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 3 -nitrotoluene to Pimephales promelas was performed according to

the US-EPA 1976 (EPA-660/3-76-097). For a test period of 48 hours an LC50 value

of 30 mg/l was reported (Mattson, 1976).