Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given (comparable to guidelines/standards)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Principles of method if other than guideline:
NEN 6501: Determination of acute toxicity with Daphnia magna (1980) with slight modifications (Van Leeuwen et al. 1985).
48 h-LC50 value and the 95 % confidence interval were calculated according to Litchfield JT and Wilcoxon F (1949).
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
1-methyl-3-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: > 98 %
- Molecular weight: 137.14

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
For each concentration, 10 daphnids were used, one animal per jar containing 50 ml medium.
The daphnids were fed on 1x10E+8 cells/L unicellular green algae (C. pyrenidosa).

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
7.5 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL; (5.6-10)

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 3 -nitrotoluene to the invertebrate Daphnia magna was performed according to

the Dutch Standardization Organization Method NEN 6501. For a test period of 48 hours an LC50 value

of 7.5 mg/l was reported (Maas-Diepeveen and van Leeuwen, 1986).