3-nitrotoluene

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

NEN 6502: Determination of chronic toxicity to Daphnia magna (1980)

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3-Nitrotoluene
- Analytical purity: > 98%

Details on test solutions:

synthetic test medium (NPR, 1980)

Test organisms (species):

Daphnia magna

Details on test organisms:

< 24 h old

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Hardness:

200 mg/l as CaCO3

Test temperature:

20 ± 0.5

pH:

8.4 ± 0.1

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

1.8 mg/L

Basis for effect:

growth

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

3.21 mg/L

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

8.3 mg/L

Two additional chronic effects presented:
1. Population growth:
LRCT(Rm) = Lowest rejected concentration (µmol/l) tested that significantly (p<0.01) lowered the population growth constant (Rm) after 21 days of exposure.
log LRCT(Rm) = 1.37
2. Body length:
LRCT(L) = Lowest rejected concentration (µmol/l

tested that significantly (p< 0.01) lowered the mean length (L) of animals after 21 days of exposure.
log LRCT(L) = 1.12

Executive summary:

Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). All daphnids used were < 24 h old at the start of the experiments. The study reports the following effect concentrations:

log (LC50): 1.78 corresponds to 60.255 µmol/L; 8.3 mg/L

log (LOEC reproduction): 1.37 corresponds to 23.44 µmol/L; 3.21 mg/L

log (LOEC growth): 1.12 corresponds to 13.18 µmol/L; 1.8 mg/L

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

in analogy with the OECD 202 proposal 1979.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: 99.5 %

Analytical monitoring:

yes

Details on test solutions:

- Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnias were 1 day old at start of incubation
- Food during incubation: Chlorella
- 25 organisms per 1 litre of test medium, 2 replicates

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

- Incubation temperature 19 ± 1 °C

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

3.5 mg/L

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

9 mg/L

Basis for effect:

other: mortality + behaviour

Executive summary:

The toxicity of 3-nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.

Description of key information

For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".

The toxicity of 3-nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.

Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). All daphnids used were < 24 h old at the start of the experiments. The study reports the following effect concentrations:

log (LC50): 1.78 corresponds to 60.255 µmol/L; 8.3 mg/L

log (LOEC reproduction): 1.37 corresponds to 23.44 µmol/L; 3.21 mg/L

log (LOEC growth): 1.12 corresponds to 13.18 µmol/L; 1.8 mg/L