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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication/study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
1-methyl-3-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: > 98 %
- Molecular weight: 137.14

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
The stock solution of the test substance was prepared in dimethylsulfoxide (DMSO, Merck, Purity 99%)

Test organisms

Test organisms (species):
Chlorella pyrenoidosa

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: reduction of the maximum density (yield)
Remarks on result:
other: 95% CL; 10-19

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 3 -nitrotoluene to the alga Chorella pyrenoidosa was performed according to

the OECD Guideline 201 (Alga, Growth Inhibition Test). For a test period of 96 hours an EC50 value

of 14 mg/l was reported (Maas-Diepeveen and van Leeuwen, 1986).