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Data on aquatic effects of thymol are available on three trophic levels (fish, daphnia and algae) for short-term toxicity and on two trophic levels (daphnia and algae) for long-term toxicity. The most sensitive short-term toxicity was observed for fish and daphnia with 96h-LC50 for fish of 4.67 mg/L and 48h-EC50 for daphnia of 4.46 mg/L, respectively. The most sensitive long-term effect was to algae with 72h-NOEC of 1.9 mg/L. Meanwhile a 3h-EC50 of 39.6 mg/L to activated sludge was also reported as the toxicity of thymol to microorganisms.

The effect concentrations of the key results reported in this chapter are nominal concentrations. The test substance concentrations were confirmed to be stable within +/- 20% of initial nominal concentration via analytical measurement. Although the test concentrations of thymol were not analytically confirmed in most of the supporting studies, the nominal concentrations are also expected to remain constant throughout the experiments as thymol is proven by the analytical determination in the key studies to be stable and no other fate or loss of the substance is expected.