REAKTIV-ORANGE DYPR 1410

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January 1999 to 28 January 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The single test concentration were analysed at the beginning and end of the study.
Test concentration : 100 mg/L
Static system in accordance with study method

Vehicle:

no

Details on test solutions:

The single test solution was dissolved into the test water media and utilised for dosing.
Method of administration : direct weighing
Solubilizer or other auxiliaries : none
Control : M4-Medium accordingto Elendt and BGA (1992)
Water for dilution: M4-Medium according to Elendt and BGA (1992)

Test organisms (species):

Daphnia magna

Details on test organisms:

Species : Daphnia magna STRAUS, parthenogenetic females
Origin of species : cloned at the Federal Health Office (BGA) in Berlin
Test animals : laboratory bred at Bayer AG Leverkusen
Age of test animals : 0-24 hours
Keeping conditions : SOP 2030-7700203-98 D of the testing laboratory.
Keeping water : M4-Medium according to Elendt and BGA (1992)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure period

Hardness:

16.3 mg CaCO3/L

Test temperature:

20 deg C

pH:

7.9

Dissolved oxygen:

8.5mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

100 mg/l (nominal)

Details on test conditions:

Operating conditions : static system in accordance with study method.
Test procedure : - cylindrical test vessels
diameter 4.0 cm, height 6.5 cm
20 ml test medium
not ventilated
No. parallel solutions : 2
Test volume : 20 ml
No- daphnids per vessel : 10

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 118 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Equates to >= 86 mg/l of organic components.

Details on results:

The above test result refers to a concentration of the test substance, which was calculated directly from analytically determined TOC values. According to the relevant product informations, 1 mg/l TOC equals to 2.6 mg/l of the organic components of the test substance (structural formula: C18H15Na2N309S3; molecular weight: 559 g/mol).
As these organic components correspond to 73 % of molecular weight of the the test substance 100 mg/l of the test substance equals to 28.1 mg/1 of organic C.

Results with reference substance (positive control):

Not applicable.

Reported statistics and error estimates:

None reported.

Results:

after 24 hours nominal concentration	after 48 hours - arithmetic mean of analytical values
EC0> 100 mg/l	EC0>86 mg/l(organic components) EC0 >118 mg/1 (test substance including inorganic components)

Validity criteria fulfilled:

yes

Conclusions:

Toxicity to daphnia (mg/l):

Exposure time : 48 h
EC0 : ≥ 100 mg/l
EC0 : ≥ 86 mg/l (organic compounds) arithmetic mean of analytical values

Executive summary:

Procedure:  "Acute Toxicity for Daphnia" council Directive 92/69/EEC C.2. Study conducted in compliance with GLP.

 

Toxicity to daphnia (mg/l):

Exposure time : 48 h                        

EC0 : ≥  86  (organic compounds)arithmetic mean of analytical values

LC0 overall: >=100 mg/l

 

The test results indicate no hazard potential. The substance is not considered to be harmful to Daphnia.

Description of key information

LC0/EC0 overall: >=100 mg/l 
The test results indicate no hazard potential. The substance is not considered to be harmful to Daphnia.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L

Additional information

Procedure:  "Acute Toxicity for Daphnia" council Directive 92/69/EEC C.2. Study conducted in compliance with GLP.

 

Toxicity to daphnia (mg/l):

Exposure time : 48 h                        

EC0 : ≥  86  (organic compounds)arithmetic mean of analytical values

LC0 overall: >=100 mg/l

 

The test results indicate no hazard potential. The substance is not considered to be harmful to Daphnia.