Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 432-080-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 October 1998 to 14 October 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- Methodology of Commission Directive 88/302/EEC, Official Journal of the EC L 133 , Part C: Biodegrad ability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209).
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Details on sampling:
- Test concentrations (mg/l): 100, 1000, 10 000
Type of application: direct weighing - Vehicle:
- no
- Details on test solutions:
- The study is carried out with nominal weights.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Test organism
Type: activated sludge (mixed population of different microorganisms)
Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority)
Pre-treatment: none
pH of the suspension before application: 7.0 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- No data
- Test temperature:
- 20.2-20.4
- pH:
- 7.9-8.1
- Dissolved oxygen:
- 5.9-6.2-start of study
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test concentrations (mg/l): 100, 1000, 10 000
- Details on test conditions:
- Test concentrations (mg/l):100, 1000, 10 000
** Test substance concentration in physico-chemical oxygen consumption control (mg/I): 10 000
Type of application: direct weighing
Test concentration of the activated sludge: 320 mg/l
Test temperature: 20 ±2⁰C
Incubation time: 3 hours with permanent aeration - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
The physico chemical oxygen consumption is determined at 10 000 mg/l test substance. No physico chemical oxygen consumption has been determined. Therefore lower concentrations of the test substance cause no physico chemical oxygen consumption (deduced values). - Results with reference substance (positive control):
- Details under any other information. The reference substance performed as expected.
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- Toxicity of Reaktiv-Orange DYPR 1410 to activated sludge:EC 50 > 10000 mg/1
- Executive summary:
Study conducted to Commission Directive 88/302/EEC, Official Journal of the EC L 133 , Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209). Performed in compliance with the Principles of Good Laboratory Practice (GLP).
A defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured. This rate is compared to those measured in test preparations with various concentrations of the test substance. In order to check the sensitivity of the activated sludge used, 3.5-dichlorophenol is used as a reference substance.
Toxicity of Reaktiv-Orange DYPR 1410 to activated sludge:EC 50 > 10000 mg/l.
The substance is not harmful to sewage sludge organisms.
Reference
Measured values of test substance / control/ reference substance
|
Test concentration [mg/l] |
Initial concentration [mg/250ml] |
O2-start
[mg O2/l] |
O2-end
[mg O2/l] |
Time [min.] |
Temp.
[C⁰] |
pH |
Test substance |
100 |
25 |
6.3 |
3.0 |
6 |
20.4 |
8.1 |
1000 |
250 |
6.4 |
2.5 |
7 |
20.3 |
8.1 |
|
10 000 |
2500 |
6.2 |
3.0 |
6 |
20.4 |
7.9 |
|
Control 1 |
|
|
5.9 |
2.6 |
6 |
20.2 |
8.1 |
Control 2 |
|
|
6.5 |
2.8 |
7 |
20.2 |
8.1 |
Physio-chemical oxygen consumption control |
10 000 |
2 500 |
8.9 |
8.9 |
9 |
20.5 |
7.4 |
Reference substance |
5 |
2.5 |
6.2 |
2.8 |
7 |
20.0 |
8.0 |
10 |
5 |
7.0 |
5.0 |
7 |
20.1 |
7.9 |
|
20 |
10 |
8.7 |
7.6 |
9 |
20.3 |
8.3 |
STUDY EVALUATION-Test substance: Reaktiv-orange DYPR 1410
Test concentration
[mg/l] |
Respiratory rate test substance
[mg/l . h] |
Phys. chem. O2-consumption
[mg/l . h] |
Respiratory rate-phys. chem. O2-consumption [mg/l . h] |
Inhibition |
100 |
33.0 |
0.0 |
33.0 |
0.0 |
1000 |
33.4 |
0.0 |
33.4 |
0.0 |
10 000 |
32.0 |
0.0 |
32.0 |
1.2 |
Control, mean |
32.4 |
|
||
Control 1 |
33.0 |
|||
Control 2 |
31.7 |
STUDY EVALUATION-Reference substance: 3.5-Dichlorophenol
Test concentration [mg/l] |
Respiratory rate [mg/l . h] |
Inhibition [%] |
5 |
29.1 |
10.2 |
10 |
17.1 |
47.2 |
20 |
7.3 |
77.5 |
Description of key information
Toxicity of Reaktiv-Orange DYPR 1410 to activated sludge:EC 50 > 10000 mg/L. The substance is not harmful to sewage sludge organisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
Study conducted to Commission Directive 88/302/EEC, Official Journal of the EC L 133 , Part C: Biodegradability: Test for inhibition of oxygen consumption (corresponds for the most part to the test method OECD 209). Performed in compliance with the Principles of Good Laboratory Practice (GLP).
Toxicity of Reaktiv-Orange DYPR 1410 to activated sludge:EC 50 > 10000 mg/L. The substance is not harmful to sewage sludge organisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
