1,8-naphthylenediamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Four groups of 5 male and 5 female Wistar rats were nose/head-only exposed to an aerosol concentration of 1,8-naphthylendiamine at an analytical concentration of 0, 156, 413 or 877 mg/m³ (highest technical feasible concentration) for 4 hours. The animals were observed for mortality, weight and clinical signs through day 14. A gross necropsy was performed.

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

1,8-naphthylenediamine

EC Number:

207-529-8

EC Name:

1,8-naphthylenediamine

Cas Number:

479-27-6

Molecular formula:

C10H10N2

IUPAC Name:

naphthalene-1,8-diamine

Details on test material:

purity: 99.5%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Vehicle:

other: acetone-polyethylenglycol 400 in air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

0. 156, 413 or 877 mg 1,8-naphthylendiamine/m³ air

No. of animals per sex per dose:

5 male and 5 female rats/concentration

Control animals:

yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 877 mg/m³ air (analytical)

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: highest technical feasible concentration

Any other information on results incl. tables

An exposition up to 877 mg/m³ 1,8-naphtylenediamine (highest technical feasible concentration) was tolerated without mortality. All animals exposed to 413 and 877 mg/m³ and revealed at the first exposition day a reduced motility and a bloody nose, additional one rat exposed to 413 mg/m³ a reduced respiration rate. All rats had no clinical symptoms at the first post-observation day. At the 3rd post-observation day in all rats exposed to 413 mg/m³ and in female rats exposed to 877 mg/m³ a reduction of weight gain was observed.

Applicant's summary and conclusion

Executive summary:

Four groups of 5 male and 5 female Wistar rats were nose/head-only exposed to an aerosol concentration of 1,8-naphthylendiamine at an analytical concentration of 0, 156, 413 or 877 mg/m³ (highest technical feasible concentration) for 4 hours. The animals were

observed for mortality, weight and clinical signs through day 14. A gross necropsy was performed.

An exposition up to 877 mg/m³ 1,8-naphtylenediamine (highest technical feasible concentration) was tolerated without mortality. All animals exposed to 413 and 877 mg/m³ and revealed at the first exposition day a reduced motility and a bloody nose, additional one rat exposed to 413 mg/m³ a reduced respiration rate. All rats had no clinical symptoms at the first post-observation day. At the 3rd post-observation day in all rats exposed to 413 mg/m³ and in female rats exposed to 877 mg/m³ a reduction of weight gain was observed. Therefore the LC50 is > 877 mg/kg bw for male and female rats