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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The highest toxicity to fish for fossil gasoline fuel (CAS No. 64741-70-4). The 96 h LL50 (mortality) to Oncorhynchus mykiss: 10 mg/l with 95 % CI:  4.5-23 mg/l (OECD 203; GLP)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
10 mg/L

Additional information

There are no acute toxicity studies on fish available for the substance. The testing was considered unjustified because there is enough evidence on acute toxicity to fish available from similar fossil gasoline fuels. The read-across data was also used to avoid unnecessary animal testing. Read-across justifications are presented in annex 1 of the CSR. The read-across approach was justified because the fate and ecotoxicological properties of renewable hydrocarbons of wood origin (napthta type fraction) are considered similar to fossil diesel fuels based on the similar composition and physical-chemical properties.

In a read-across study from CONCAWE (1995, study no 104558) determined acute toxicity of isomerate gasoline sample W94/810 CAS No. 64741-70-4 Naphtha (petroleum) isomerization was determined with an OECD 203 96-hour Oncorhynchus mykiss test complying with GLP. The study was conducted as WAF loading rates. The 96-hour LL50 was calculated to be 10 mg/L with a 99% confidence interval of 4.5 to 23 mg/L.

In a read-across study from CONCAWE (1995, study no 104858) determined acute toxicity of blended gasoline sample (TS MRD-95-048, blended gasoline sample W94/813, CAS No. 86290-81-5) was determined with an OECD 203 96-hour Oncorhynchus mykiss test complying with GLP. The study was conducted as WAF loading rates. The 96-hour LL50 was calculated to be 11 mg/L.

In a read-across study from CONCAWE (1995, study no 104958) determined acute toxicity of blended gasoline sample (TS MRD-95-049, blended gasoline sample W94/814, CAS No. 86290-81-5) was determined with an OECD 203 96-hour Oncorhynchus mykiss test complying with GLP. The study was conducted as WAF loading rates. The 96-hour LL50 was calculated to be 16 mg/L.

In conclusion, the key value for CSA was 10 mg/L (CONCAWE 1995, study no 104558). It was selected because it was the highest toxicity response from a study that was done according to OECD standards and complied with GLP.