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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 2000-12-13 to 2001-04-27
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Remarks:
The study was conducted in accordance with "Eco-toxicity test Standards" by Japanese Ministry of the Environment.

Test material

Constituent 1
Reference substance name:
3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide
EC Number:
229-245-3
EC Name:
3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide
Cas Number:
6448-95-9
IUPAC Name:
3-hydroxy-4-[(2-methyl-5-nitrophenyl)diazenyl]-N-phenyl-2-naphthamide

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
No

Test conditions

Hardness:
Total hardness 63.1 mg/L
Test temperature:
Temperature: 20 +/- 1 deg C
pH:
8.0 to 8.1
Dissolved oxygen:
8.6 to 8.9 mg/L
Nominal and measured concentrations:
Nominal limit concentration: 100 mg/L;
Measured concentration test start: 100 mg/L;
Measured concentration test end (48 hours): 120 mg/L;
Geometric mean measured concentration: 110 mg/L.
The concentration of test substance in the control and solvent control were less than the quantification limit (0.06 mg/L).
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 110 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
110 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 110 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
No statistical analysis was applied for the calculation of median effective concentration (EC50), since the study was conducted as a limit test at 100 mg/L.
The no-observed-effect concentration (NOEC) was defined as the highest concentration where no adverse effect on daphnids was observed, and the lowest
concentration producing 100% immobilization was defined as the lowest concentration (EC100) where all daphnids were observed immobilized.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes