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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08/09-1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Principles of method if other than guideline:
Toxizitaetstest fuer Regenwuermer in künstlichem Boden nach 88/302 EWG
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Reaction products of coconut oil with polyethyleneglycol and 2-ethyl-2-(hydroxymethyl)propane-1,3-diol
EC Number:
640-964-5
Cas Number:
218451-68-4
Molecular formula:
not applicable because UVCB
IUPAC Name:
Reaction products of coconut oil with polyethyleneglycol and 2-ethyl-2-(hydroxymethyl)propane-1,3-diol
Details on test material:
- Name of test material (as cited in study report): Marlowet 4753
- Physical state: liquid, cristalline
- Stability under test conditions: > 1 year

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil: The test substance was mixed with silica sand and afterwards added to soil substrate in a plastic bag. Water was added to a theoretical water content of approx. 40% and the substrate was thoroughly mixed
pH = 6

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH&Co. Produktions- und Vertrieb KG, Kapellenstraße 25, Wiesbaden, Germany
- Age at test initiation: at least 2 months
- Weight at test initiation: average 350 mg (control), average 354 mg (test substance)

ACCLIMATION
- Acclimation period: not mentioned

Study design

Study type:
laboratory study
Substrate type:
other: peat and dried horse dung
Limit test:
yes
Total exposure duration:
14 d
Post exposure observation period:
no

Test conditions

Test temperature:
20 +- 2 °C
pH:
6
Moisture:
40 %
Details on test conditions:
TEST SYSTEM
- Test container (material, size): Makrolon cages with perforated plexiglass covers, size not mentioned
- Amount of soil or substrate: not mentioned
- No. of organisms per container (treatment): 10

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): mixture of peat and horse dung
- Organic carbon (%): not mentioned
- Maximum water holding capacity (in % dry weigth): 40 %
- CEC: not mentioned
- Pretreatment of soil: not mentioned
- Storage (condition, duration): fresh prepared
- Stability and homogeneity of test material in the medium: not mentioned

OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity: approx. 400 lux

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Range finding study: not performed
Nominal and measured concentrations:
not measured; nominal concentration 1000 mg/kg dry soil
Reference substance (positive control):
yes
Remarks:
chloracetamide, historical data

Results and discussion

Effect concentrations
Key result
Duration:
14 d
Dose descriptor:
LC10
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: 1
- Total mass of adults at beginning of test: 14 g (test substance), 14.16 g (control), 40 adults each
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: not mentioned
- No. of offspring produced: not mentioned
- No. of unhatched cocoons: not mentioned
- Morphological abnormalities: not mentioned
- Behavioural abnormalities: not mentioned
- Other biological observations: not mentioned
Results with reference substance (positive control):
RESULTS: TEST WITH REFERENCE SUBSTANCE (historical data, from 1996-03-13 to 1996-03-27)
- Concentrations: 0, 10, 30 and 100 mg 2-chloroacetamide/kg dry soil
- Results (mortality): 0, 10, 30 mg/kg: 0/20 (0%) after 7 and 14 days, respectively; 100 mg/kg: 20/20 (100%) after 7 and 14 days, respectively.

Any other information on results incl. tables

RESULTS: EXPOSED

- Nominal concentrations: 1000 mg/kg dry soil

- Effect data (Mortality): 0/40 (0%) after 7 days; 1/40 (2.5%) after 14 days

RESULTS: CONTROL

- Effect data (Mortality): 0/40 (0%) after 7 and 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

In the toxicity limit test with Eisenia foetida foetida the test substance Marlowet 4753 (Coconut oil, reaction products with polyethylene glycol and trimethylolpropane) showed at a concentration of 1000 mg/kg (<= 10 %) no mortality after 7 and 14 days.