Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 629-720-9 | CAS number: 1219826-66-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine
- EC Number:
- 219-145-8
- EC Name:
- N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine
- Cas Number:
- 2372-82-9
- Molecular formula:
- C18H41N3
- IUPAC Name:
- N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Moisture:
- 1.5%
- Details on test conditions:
- - Following a preliminary range-finding test (nominal concentrations of 1.0, 10, 100 and 1,000 mg/kg), 18 soil micro-organism (Mixed population of soil microorganisms) test samples were exposed to a concentration of 1,000 mg/kg for a period of 28 d at a temperature of approximately 21°C.
- On Days 0, 7 and 28 triplicate control and six replicate test substance vessels were sacrificed for nitrate analysis. - Nominal and measured concentrations:
- Nominal concentration: 1,000 mg a.i./kg soil dw
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- nitrate formation rate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- nitrate formation rate
- Details on results:
- Range-finding study:
- The results showed no significant effect on nitrogen transformation activity.
- The absorbance values obtained for the 1,000 mg a.i./kg soil dw test concentration on day 0 were significantly higher than those obtained for the control an 1, 10 and 100 mg a.i./kg soil dw test concentrations. The reason for this could not be determined from the data however, as the results of the analyses conducted on day 28 confirmed that the test substance exhibited no adverse effect on the nitrogen transformation rate, it was considered that these relatively high results did not affect the outcome. Based on this result a single test concentration of 1,000 mg a.i./kg soil dw was selected for the definitive test.
Definitive study:
- The 28-d EC50 was estimated to be > 1,000 mg a.i./kg soil dw, where EC50 is the test concentration that reduced soil nitrogen transformation activity by 50 %.
- The test substance vessels were shown to have higher nitrate concentration than the control vessels on days 7 and 14 of the test. This increase in nitrate concentration was also observed in the range-finding test. The test substance was known to adsorb to soil/sediment and data provided by the Sponsor indicated that the test substance was readily biodegradable. It was therefore considered likely that under the experimental conditions employed in this study the test substance would adsorb to the soil with small amounts being present in the soil moisture. The small amount of test substance present in the soil moisture would then be available for biodegradation by the soil micro-organisms present. In the presence of a readily degradable carbon source available for growth and respiration, the numbers of soil micro-organisms present would increase at a faster rate than that in the control vessels which in turn would lead to an increase in the nitrogen transformation rate of the added respiratory substrate (powdered Lucerne-grass-green-meal).
- As the increase in nitrate concentrations in the test substance group could have only been due to an increase in the nitrogen transformation rate it was considered that the test substance exhibited no adverse effect on the nitrogen transformation rate and therefore the "No Observed Effect Concentration" (NOEC) was 1,000 mg a.i./kg soil dw. Further, it was considered unnecessary and unrealistic to test at concentrations in excess of 1,000 mg a.i./kg soil dw. - Reported statistics and error estimates:
- - Statistical analysis of the nitrate concentration values was carried out for the control and test substance group using a Student’s t-test. The test substance group was shown to have significantly higher nitrate concentrations than the control group (P = 0.05).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, there was no significant effect of the test substance on the nitrogen transformation activity of soil microorganisms and therefore the 28 days EC50 value was estimated to be >1000 mg a.i./kg soil dw and the corresponding NOEC was 1000 mg a.i./kg soil dw.
- Executive summary:
A study was conducted to determine the effect of the test substance on the nitrogen transformation activity of soil microorganisms according to the OECD Guideline 216, in compliance with GLP. Following a preliminary range-finding test (nominal concentrations of 1, 10, 100 and 1000 mg a.i./kg soil dw), 18 soil micro-organism (mixed population of soil microorganisms) test samples were exposed to a concentration of 1000 mg a.i./kg soil dw for a period of 28 d at a temperature of approximately 21°C. On Days 0, 7 and 28, triplicate control and six replicate test substance vessels were extracted for nitrate analysis. The test vessels were shown to have higher nitrate concentration than the control vessels on Days 7 and 14 of the test. Under the study conditions, there was no significant effect of the test substance on the nitrogen transformation activity of soil microorganisms and therefore the 28 d EC50 value was estimated to be >1000 mg a.i./kg soil dw and the corresponding NOEC was 1000 mg a.i./kg soil dw (Mead, 2004).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
