4'-ethoxy-3-hydroxy-2-naphthanilide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study was carried out according to test guidline and under GLP-conditions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4'-ethoxy-3-hydroxy-2-naphthanilide

EC Number:

225-200-7

EC Name:

4'-ethoxy-3-hydroxy-2-naphthanilide

Cas Number:

4711-68-6

Molecular formula:

C19H17NO3

IUPAC Name:

N-(4-ethoxyphenyl)-3-hydroxy-2-naphthamide

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

2.3 - 2.5 mmol Ca2+ + Mg2+/L

Test temperature:

21.5 - 22-3

pH:

7.7 -7.9

Dissolved oxygen:

7.2 - 8.6 mg/L

Nominal and measured concentrations:

0/10000 mg/L (nominal)
0/0.2 mg/L (mean of measured conc.)

Details on test conditions:

use of reconstituted water, composition acc. to ISO/DIS 7346/1

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.2 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.2 mg/L

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Remarks on result:

other: not determined

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

67/548/EEC: Acute aquatic toxicity: no classification
1272/2008/EC: Acute aquatic toxicity: no classification

Executive summary:

No lethality was observed up to the solubility limit of Naphtol AS-VL. The 10000 mg/L (0.2 mg/L*) group exhibited reversible symptoms.

* = value based on the mean of analytically determined values