α,α,α-trifluoro-o-toluidine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

other: Although less details on test conditions and on analytical method endpoint results are used for classifation

Rationale for reliability incl. deficiencies:

other: Not enough details on test conditions, no analytical method.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: AFNOR norm T 90301 of april 1974, modified according to AFNOR doc T 95 B for reconstitued river water

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trifluoro-o-toluidine

EC Number:

201-806-7

EC Name:

α,α,α-trifluoro-o-toluidine

Cas Number:

88-17-5

Molecular formula:

C7H6F3N

IUPAC Name:

2-(trifluoromethyl)aniline

Constituent 2

Reference substance name:

alpha,alpha,alpha-trifluoro-o-toluidine

IUPAC Name:

alpha,alpha,alpha-trifluoro-o-toluidine

Details on test material:

o-TFMA used was the commercial quality.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Preparation and application of test solution:
The test substance was diluted in bidistilled water at a concentration slightly higher than expected toxicity treshold.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

no data

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Details on test conditions:

WATER PARAMETERS
- Source/preparation of dilution water: reconstituted river water

Other test conditions: no data

Reference substance (positive control):

no

Remarks:

preliminary tests were done to determine level of toxicity in the main test

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

10 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 8-12 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 24h was 10 mg/L based on mobility. 
Therefore, the EC50 48h is between 1 and 10 mg/l, and the substance considered as toxic to aquatic organisms.

Since the substance is not readily biodegradable it is classified as:

Directive 67/548/EEC: R51/R53
Regulation (EC) No. 1272/2008: H411

Executive summary:

The ortho-trifluoromethylaniline has been tested for acute toxicity to Daphnia magna Strauss (strain IRCHA) according to the AFNOR norm T 90301 of april 1974.

The daphnids, obtained from the laboratory breeding, were assayed during 24 hours, in the dark, at 20 +/-1 °C, in a range of substance concentrations prepared in the assay medium (20 daphnids per concentration).

The EC50 24h was 10 mg/L based on mobility.

 

Therefore, the EC50 48h is between 1 and 10 mg/l, and the substance considered as toxic to aquatic organisms.