2,4,6-trimethyl-1,3,5-trioxane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD Guideline 202 and GLP Standard.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Phase I

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trimethyl-1,3,5-trioxane

EC Number:

204-639-8

EC Name:

2,4,6-trimethyl-1,3,5-trioxane

Cas Number:

123-63-7

Molecular formula:

C6H12O3

IUPAC Name:

2,4,6-trimethyl-1,3,5-trioxane

Test material form:

other: liquid

Details on test material:

Name for report: P0087
Batch-No.: TK 1-19.9.89
Purity: 99.7 %
Solubility in water 120 g/L

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

356 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity study on invertebrates (Daphnia magna) the 48 hour EC50 was determined to be 356 mg/L.

Executive summary:

A static acute toxicity test with Daphnia magna was performed in year 1990 according to OECD Guideline 202 and GLP.

No immobilisation or effects on the Daphnia were noted neither after 24 h nor after 48 h. The pH of the test solutions (8.1), the oxygen concentrations and temperature of the test solutions were within acceptable limits (Oxygen concentration: 80 - 100 % saturation, temperature: 20.8 - 21.9 °C).

The 48 hour EC50 was determined to be 356 mg/L.