Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to UBA-method to determine the toxic potential of chemical substances for water systems, Sept. 1979, Heft 10, federal environmental agency and GLP Standard.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: UBA-method, Heft 10, federal environmental agency
Principles of method if other than guideline:
SOP MIB/M/31 Determination of biological effects of toxic substances against bacteria.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
Name used for report: P0087
Batch No.: Tk1-19.9.89
Purity: 99.7%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
18 h

Results and discussion

Effect concentrations
Duration:
18 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In the presence of test item P0087, starting with a concentration of 1000 mg/L and below, no inhibition of growth for Pseudomonas putida could be
detected. Therefore, the EC10 (starting concentration) after 18 h incubation is > 1000 mg/L.
Executive summary:
A microorganisms test with Pseudomonas putida as test species was performed in year 1990 according to UBA-method to determine the toxic potential of P0087 for water systems. In the presence of test item P0087, starting with a concentration of 1000 mg/L and below, no inhibition of growth for Pseudomonas putida could be detected. Therefore, the EC10 (starting concentration) after 18 h incubation is > 1000 mg/L.