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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Key value for chemical safety assessment

Additional information


In a reverse gene mutation assay in bacteria (BASF SE, 2009), strains TA 98 and TA mix (i.e. TA7001, TA 7002, TA 7003, TA 7004, TA 7005 and TA 7006) of S. typhimurium were exposed to 2-Methyl-3,4-dibrombuttersäureethylester (ca. 98% pure) in DMSO at concentrations of 4, 20, 100, 500, 2500, and 5000 µg/ml in the presence and absence of mammalian metabolic activation (liquid suspension assay; Ames II Assay). The test was carried out according to a modified version of the Ames test (OECD 471), designated Ames II Assay (microtiter version).

2-Methyl-3,4-dibrombuttersäureethylester was tested up to limit concentrations. The positive controls induced the appropriate responses in the corresponding strains.

There was no evidence of induced mutant colonies over background.

This study is classified as acceptable. This study satisfies the requirement for Test Guideline OECD 471 for in vitro mutagenicity (bacterial reverse gene mutation) data.


No data is available.

Short description of key information:
Not mutagenic in bacteria (Ames II Assay) (BASF SE, 2009).
No classification / labeling required.

No data is available.

Endpoint Conclusion:

Justification for classification or non-classification

There is no need to classify 2-Methyl-3,4-dibrombuttersäureethylester for mutagenicity according to the Directive 67/548/EC and according to the GHS criteria.