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EC number: 257-515-0 | CAS number: 51920-12-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- see Read-across justification in section 13 for details
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L. All effect levels are given based on the nominal concentration of the test item.
- Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4910 cells/mL. A saturated solution* with a nominal concentration of 1 mg/L was tested. Six replicates were tested for the limit concentration and the control. Environmental conditions were determined to be within the acceptable limits.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition
NOEC
1.00
LOEC
> 1.00
ErC10
> 1.00
ErC20
> 1.00
ErC50
> 1.00
Inhibition of Yield
NOEC
1.00
LOEC
> 1.00
EyC10
> 1.00
EyC20
> 1.00
EyC50
> 1.00
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-12-09 to 2011-12-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to relevant guidelines and compliant to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- according to German Chemikaliengesetzt and directive 88/320/EEC
- Analytical monitoring:
- no
- Details on sampling:
- As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1 mg/L was freshly prepared with dilution water. The stock solution was stirred for 48 hours (1100 rpm) with a magnetic stirrer. Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL).
- Eluate: Dilution water
- Differential loading: None- limit test concentration
- Controls: Six replicates (without test item) were tested under the same test conditions as the test replicates. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Dilution water: 0.24 mmol Ca+mg/L.
- Test temperature:
- min.: 21.5 max.: 22.0, mean value: 21.8
- pH:
- Nominal concentration pH-value
[mg/L] Start; 0 hours End; 72 hours
1.00 8.01 8.51
Control 8.09 8.56 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 103 cells/mL, Actual: mean 4910 cells/mL
- Control end cells density: Mean 614231 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1, mean value: 69.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
TEST CONCENTRATIONS
- Range finding study: No
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
EC50 Values of the Reference Item
based on nominal concentrations (mg/L), (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Rate-related inhibition
ErC50 0.488 0.699 ± 0.471
95 % confidence interval 0.466 – 0.512
Yield inhibition
EyC50 0.291 0.318 ± 0.147
95 % confidence interval 0.274 – 0.328 - Reported statistics and error estimates:
- NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. As standard One Way Analysis of Variance (ANOVA) and DUNNETT’s Test were used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L.
The test item caused a slight stimulation on the algae growth. All effect levels are given based on the nominal concentration of the test item. - Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4910 cells/mL. A saturated solution* with a nominal concentration of 1 mg/L was tested. Six replicates were tested for the limit concentration and the control. Environmental conditions were determined to be within the acceptable limits.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition
NOEC
1.00
LOEC
> 1.00
ErC10
> 1.00
ErC20
> 1.00
ErC50
> 1.00
Inhibition of Yield
NOEC
1.00
LOEC
> 1.00
EyC10
> 1.00
EyC20
> 1.00
EyC50
> 1.00
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-12-02 to 2011-12-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to relevant guidelines and compliant to GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- according to German Chemikaliengesetzt and directive 88/320/EEC
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 1 mg/L was freshly prepared with dilution water. The test item was weighed out and stored overnight at room temperature in the dark before the preparation of the stock solution. The stock solution was stirred for 48 hours (1100 rpm) with a magnetic stirrer. Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, Macherey-Nagel). Centrifugation for 20 min at 10000 rpm was found to be insufficient.
- Eluate: Dilution water
- Differential loading: Saturated solution* corresponding to a nominal test item concentration of 1.00 mg/L
- Controls: Six replicates (without test item) were tested under the same test conditions as the test replicates. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
- Hardness:
- Dilution water: 0.24 mmol Ca+mg/L.
- Test temperature:
- min.: 21.5 max.: 22.0, mean value: 21.8
- pH:
- Nominal concentration pH-value
[mg/L] Start; 0 hours End; 72 hours
1.00 7.66 8.20
Control 7.91 8.24 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 103 cells/mL, Actual: mean 4910 cells/mL
- Control end cells density: Mean 996626 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1, mean value: 69.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
TEST CONCENTRATIONS
- Range finding study: No
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
EC50 Values of the Reference Item
based on nominal concentrations (mg/L), (0-72 hours)
Current Study Valid Range (average ± 3 x SD)
Rate-related inhibition
ErC50 0.488 0.699 ± 0.471
95 % confidence interval 0.466 – 0.512
Yield inhibition
EyC50 0.291 0.318 ± 0.147
95 % confidence interval 0.274 – 0.328 - Reported statistics and error estimates:
- NOEC/LOEC was determined by calculation of statistical significance of growth rates and yield. As standard One Way Analysis of Variance (ANOVA) was used for NOEC/LOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L. All effect levels are given based on the nominal concentration of the test item.
- Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4910 cells/mL. A saturated solution* with a nominal concentration of 1 mg/L was tested. Six replicates were tested for the limit concentration and the control. Environmental conditions were determined to be within the acceptable limits.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition
NOEC
1.00
LOEC
> 1.00
ErC10
> 1.00
ErC20
> 1.00
ErC50
> 1.00
Inhibition of Yield
NOEC
1.00
LOEC
> 1.00
EyC10
> 1.00
EyC20
> 1.00
EyC50
> 1.00
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- see Read-across justification in section 13 for details
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: As the test item is highly insoluble in water, no suitable method for determination of the test item could be established.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L.
The test item caused a slight stimulation on the algae growth. All effect levels are given based on the nominal concentration of the test item. - Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4910 cells/mL. A saturated solution* with a nominal concentration of 1 mg/L was tested. Six replicates were tested for the limit concentration and the control. Environmental conditions were determined to be within the acceptable limits.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition
NOEC
1.00
LOEC
> 1.00
ErC10
> 1.00
ErC20
> 1.00
ErC50
> 1.00
Inhibition of Yield
NOEC
1.00
LOEC
> 1.00
EyC10
> 1.00
EyC20
> 1.00
EyC50
> 1.00
Referenceopen allclose all
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
ErC10 |
> 1.00 |
ErC20 |
> 1.00 |
ErC50 |
> 1.00 |
Inhibition of Yield |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
EyC10 |
> 1.00 |
EyC20 |
> 1.00 |
EyC50 |
> 1.00 |
Cell Densities
|
Nominal concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
|
|||
1.00 |
1 |
4910 |
19282 |
138488 |
814696 |
|
|||
2 |
4910 |
22038 |
149838 |
793845 |
|
||||
3 |
4910 |
20575 |
132890 |
735183 |
|
||||
4 |
4910 |
17867 |
124877 |
684205 |
|
||||
5 |
4910 |
19289 |
140161 |
734099 |
|
||||
6 |
4910 |
17373 |
132524 |
771064 |
|
||||
Mean |
4910 |
19404 |
136463 |
755515 |
|
||||
Control |
1 |
4910 |
19316 |
110897 |
572059 |
|
|||
2 |
4910 |
17221 |
111083 |
635190 |
|
||||
3 |
4910 |
16815 |
131363 |
642671 |
|
||||
4 |
4910 |
18382 |
98830 |
663725 |
|
||||
5 |
4910 |
16845 |
105258 |
584550 |
|
||||
6 |
4910 |
16500 |
101927 |
587190 |
|
||||
Mean |
4910 |
17513 |
109893 |
614231 |
|
||||
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
1.00 |
1 |
1.70 |
-5.88 |
809786 |
-32.9 |
||
2 |
1.70 |
-5.35 |
788935 |
-29.5 |
|||
3 |
1.67 |
-3.76 |
730273 |
-19.9 |
|||
4 |
1.65 |
-2.27 |
679295 |
-11.5 |
|||
5 |
1.67 |
-3.73 |
729189 |
-19.7 |
|||
6 |
1.69 |
-4.74 |
766154 |
-25.7 |
|||
Mean |
(+)* |
1.68 |
-4.29 |
(+)* |
750605 |
-23.2 |
|
Control |
1 |
1.59 |
567149 |
||||
2 |
1.62 |
630280 |
|||||
3 |
1.63 |
637761 |
|||||
4 |
1.64 |
658815 |
|||||
5 |
1.59 |
579640 |
|||||
6 |
1.60 |
582280 |
|||||
Mean |
1.61 |
609321 |
|||||
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0 – 72 hours)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.37 |
1.75 |
1.64 |
1.59 |
0.195 |
12.3 |
19.4 |
2 |
1.26 |
1.86 |
1.74 |
1.62 |
0.323 |
19.9 |
||
3 |
1.23 |
2.06 |
1.59 |
1.63 |
0.414 |
25.5 |
||
4 |
1.32 |
1.68 |
1.90 |
1.64 |
0.295 |
18.0 |
||
5 |
1.23 |
1.83 |
1.71 |
1.59 |
0.318 |
19.9 |
||
6 |
1.21 |
1.82 |
1.75 |
1.60 |
0.333 |
20.9 |
||
Mean |
1.61 |
|
||||||
SD ± |
0.02 |
|
||||||
CV [%] |
1.27 |
|
||||||
NOEC, LOEC, EC - values of the test item (0-72 hours)
based on nominal concentration [mg/L]
Rate-related Inhibition |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
ErC10 |
> 1.00 |
ErC20 |
> 1.00 |
ErC50 |
> 1.00 |
Inhibition of Yield |
|
NOEC |
1.00 |
LOEC |
> 1.00 |
EyC10 |
> 1.00 |
EyC20 |
> 1.00 |
EyC50 |
> 1.00 |
Cell Densities
|
Nominal concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
|
|||
1.00 |
1 |
4910 |
29661 |
189074 |
1032501 |
|
|||
2 |
4910 |
24620 |
184362 |
987954 |
|
||||
3 |
4910 |
28527 |
189886 |
1070243 |
|
||||
4 |
4910 |
29674 |
179799 |
1133848 |
|
||||
5 |
4910 |
25670 |
187794 |
913213 |
|
||||
6 |
4910 |
25199 |
172806 |
895848 |
|
||||
Mean |
4910 |
27225 |
183954 |
1005601 |
|
||||
Control |
1 |
4910 |
26191 |
210596 |
920559 |
|
|||
2 |
4910 |
29918 |
182195 |
1080568 |
|
||||
3 |
4910 |
28659 |
201707 |
1036258 |
|
||||
4 |
4910 |
26513 |
171892 |
955289 |
|
||||
5 |
4910 |
30233 |
158419 |
998651 |
|
||||
6 |
4910 |
25781 |
180496 |
988428 |
|
||||
Mean |
4910 |
27883 |
184218 |
996626 |
|
||||
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
1.00 |
1 |
1.78 |
-0.69 |
1027591 |
-3.62 |
||
2 |
1.77 |
0.14 |
983044 |
0.87 |
|||
3 |
1.80 |
-1.37 |
1065333 |
-7.42 |
|||
4 |
1.81 |
-2.45 |
1128938 |
-13.8 |
|||
5 |
1.74 |
1.62 |
908303 |
8.41 |
|||
6 |
1.74 |
1.98 |
890938 |
10.2 |
|||
Mean |
(-) |
1.77 |
-0.13 |
(-) |
1000691 |
-0.91 |
|
Control |
1 |
1.75 |
915649 |
||||
2 |
1.80 |
1075658 |
|||||
3 |
1.78 |
1031348 |
|||||
4 |
1.76 |
950379 |
|||||
5 |
1.77 |
993741 |
|||||
6 |
1.77 |
983518 |
|||||
Mean |
1.77 |
991716 |
|||||
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0 – 72 hours)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.67 |
2.09 |
1.48 |
1.75 |
0.311 |
17.8 |
7.94 |
2 |
1.81 |
1.81 |
1.78 |
1.80 |
0.0154 |
0.859 |
||
3 |
1.76 |
1.95 |
1.64 |
1.78 |
0.158 |
8.87 |
||
4 |
1.69 |
1.87 |
1.72 |
1.76 |
0.0983 |
5.60 |
||
5 |
1.82 |
1.66 |
1.84 |
1.77 |
0.101 |
5.68 |
||
6 |
1.66 |
1.95 |
1.70 |
1.77 |
0.155 |
8.79 |
||
Mean |
1.77 |
|
||||||
SD ± |
0.02 |
|
||||||
CV [%] |
1.07 |
|
||||||
Description of key information
RA from PBr25
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L.
The test item caused a slight stimulation on the algae growth.
RA from PR176
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201.
As the test item is highly insoluble in water, no suitable method for determination of the test item could be established and no analytical determination was carried out. All effect values of the test item are given based on the nominal concentration.
In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L. All effect levels are given based on the nominal concentration of the test item.
Key value for chemical safety assessment
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
