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Oxooil LS9 has been tested for acute toxicity for three trophic levels (fish, aquatic invertebrates and algae) using water accommodated fractions (WAFs). It was not possible to measure the soluble fraction (achieved concentration), by chemical analysis. The most sensitive species for acute aquatic toxicity is Daphnia Magna with an EC50 (48 h) of 12 mg/l nominal loading rate WAF and a NOEC of 5.6 mg/l nominal loading rate WAF. Oxooil LS 9 is therefore evaluated as acutely harmful to aquatic organisms. Oxooil LS9 is not harmful to Fish or Algae. The LC50 (96 ah) for Oncorhynchus mykiss is >100 mg/l and the EC 50 (72 h) for growth rate for the green algae Desmodesmus subspicatus is 120 mg/l. It was not possible to investigate the toxicity to bacteria in an activated sludge respiratory inhibition test as it's volatility would have compromised the test result.