2-[(1-amino-9,10-dihydro-4-hydroxy-9,10-dioxo-2-anthryl)oxy]ethyl phenyl carbonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 January 2000 to 3 February 2000.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

Test concentrations:
A range-finding test with the following test concentrations was carried out: 0.01, 0.1, 1.0, 10 and 100 mg/l. Since no immobilisation of daphnids occurred in any of the treatments only the highest test concentration of a 100 mg of test item/I and a blank control was performed for the definitive test.

Vehicle:

no

Details on test solutions:

The test medium used had the following composition:
-NaHCO3: 65 mg/l
-MgSO4*7H2O: 123.3 mg/l
-KCl: 5.8 mg/l
-CaCl2*2H2O: 294 mg/l
-Na2SiO3: 4.3 mg/l
-NaNO3: 274 µg/l
-KH2P04: 143 µg/l
-K2HP04: 184 µg/l

The test medium was prepared using bidistilled water.
The pH was in the range of 7.6 - 8.0.
The test medium was oxygen saturated at the beginning of the test.

Total hardness 152 mg/l CaC03.
Ca / Mg ratio 4 : 1.
Na/K ratio 10:1.
Alkalinity 0.8 mmol/l.

Test organisms (species):

Daphnia magna

Details on test organisms:

The organism used in this test system was the species Daphnia magna Straus. The Daphnia were bred in the laboratories of the Ecotox Center under standardised conditions.

Breeding:
Cultures of Daphnia magna are held in glass vessels containing approximately 3 litres of reconstituted water at 18 - 22 °C. The water is renewed partially three times per week. The Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a Tetramin-extract in such quantities that the food is consumed within 24 hours.

Water: test medium
Light: 14 hours photoperiod daily, 10 hours darkness.
Test vessels 250 ml beakers containing 50-100 ml test medium with 10 animals per beaker. All tests were run in duplicate.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

152 mg/l CaCO3

Test temperature:

19.4 °C

pH:

7.9 (In the highest test concentration)

Details on test conditions:

Pre-treatment :
24 hours before the start of the exposure, reproductive Daphnia were separated from the young daphnids by sieving all individuals through a 1000 µm sieve.
For each test concentration 20 daphnids divided into two groups of 10 animals were used.

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

In the blank:
0 % immobility after 24 and 48 hours

At 100 mg/l:
0 % immobility after 24 and 48 hours

Results with reference substance (positive control):

The quality of the Daphnia is checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate.
Reference control October 06, 1999: EC50 0.9 mg/l.

None

Validity criteria fulfilled:

yes

Conclusions:

The EC 50 value of FAT 93504/A after 48h was determined to be greater than 100 mg/l

Executive summary:

The goal of this acute toxicity study to daphnia magna is to determine the EC50 of FAT 93504/A (Concentration at which 50 % of the Daphnia test population is immobilised). This study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna Straus were used, 20 Daphnia at each test concentrations. The percent of immobility of daphnids after 24 and 48 hours in contact with the substance was determined in this static test, at 19.4 -21 °C. The nominal concentration of FAT 93504/A tested was 100 mg test item/l. Additionally, a blank control without test item was carried out. The test concentration was prepared from a stock solution. The NOEC and the EC0 value after 48 hours was determined to be >100 mg/l. The EC50 value after 48 hours was determined as above 100 mg/l. The EC100 value after 48 hours is higher than 100 mg/l. No daphnids were immobilised in the control and in the test concentration of 100 mg/l. According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 93504/A is 'non-toxic' to Daphnia magna following an exposure period of 48 hours.

Description of key information

Concentration at which 50 % of the Daphnia test population is immobilised is greater than 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) to determine the EC50 of FAT 93504/A to Daphnia magna. The percent of immobility of daphnids after 24 and 48 hours in contact with the substance was determined in this static test, at 19.4 -21 °C. The nominal concentration of FAT 93504/A tested was 100 mg test item/l. Additionally, a blank control without test item was carried out. The test concentration was prepared from a stock solution. The NOEC and the EC0 value after 48 hours was determined to be >100 mg/l. The EC50 value after 48 hours was determined as above 100 mg/l. The EC100 value after 48 hours is higher than 100 mg/l. No Daphnia were immobilised in the control and in the test concentration of 100 mg/l. According to Directive 93/21 /EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT 93504/A is 'non-toxic' to Daphnia magna following an exposure period of 48 hours.