Retinyl propionate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, however only screening test was performed. Non GLP, no chemical analysis and no information given on the purity of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Principles of method if other than guideline:

Screening test performed according to the OECD guideline 202

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Retinol

EC Number:

200-683-7

EC Name:

Retinol

Cas Number:

68-26-8

Molecular formula:

C20H30O

IUPAC Name:

(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraen-1-ol

Details on test material:

- Name of test material (as cited in study report): Retinol crystalline
- Lot/batch No.: CP 090-03-04-57 Fi. 279
No data on test substance purity

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The highest test concentration, 100 mg test substance/l, was prepared in M4 medium and stirred for approx. 20 hours. Subsequently, the test solution was centrifuged at 17700 G for 20 minutes. Following this, serial dilutions of the aqeous extract (eluate) were prepared in order to obtain the desired test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.01, 0.1, 1, 10, 100, control

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to OECD guideline

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The number of mobile daphnids after hours of exposure in the different  test concentrations was as following: 

Cumulative immobilization of daphnia after 48 hours [%]:
Nominal test concentration  
[mg/l]       48h
  0.00    /   0   /
  0.01    /   5   /
  0.10    /   0   /
  1.00    /  10   /
 10.00    /   0   /
100.00    /  35   /