Carboxylic acids, C5-9, triesters with 2-ethyl-2-(hydroxymethyl)propane-1,3-diol

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, it meets generally accepted specific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Medium-and long-chain fatty acid uptake and utilization by Streptomyces coelicolor A3 (2): first characterization of a Gram-positive bacterial system

Author:

Banchio, C. and Gramajo, H.C.

Year:

1997

Bibliographic source:

Microbiology 143, 2439-2447

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

This study investigated the ability of Streptomyces to utilize different chain length fatty acids as sole carbon and energy sources, and to characterize their uptake system biochemically

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids of different chain length (C4-C18)

IUPAC Name:

Fatty acids of different chain length (C4-C18)

Details on test material:

Name of test material (as cited in study report): Fatty acids of different chain length (C4-C18)
Analytical purity: no data

Sampling and analysis

Analytical monitoring:

not required

Test organisms

Test organisms (inoculum):

other: Streptomyces coelicolor

Study design

Remarks:

Not applicable. In-vitro assay.

Test conditions

Details on test conditions:

EFFECT PARAMETERS MEASURED: In-vivo fatty acid degradation

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Dose descriptor:

other: Biodegradation of the test material

Effect conc.:

ca. 0.1 other: % w/v

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Biodegradation of the test material

Remarks on result:

other: S.coelicolor strain M145 utilize exogenous fatty acids of different chain lenght, from C4 to C18

Any other information on results incl. tables

The study indicated that S.coelicolor strain M145 can effectively utilize fatty acids of different chain length, from C4 to C18, as sole carbon energy source. The in vivo ß-oxidation studies in cells grown in the presence or absence of fatty acids (Table 1), and in vitroassay of two enzymes of the pathway, acyl-CoA synthetase and acyl-CoA dehydrogenase (Table 2), clearly indicate thatS. coelicolorconstitutively expressed the enzymes of the ß-oxidation cycle, without the need for the induction by a fatty acid of any chain length. The ß-oxidation pathway in this microorganism, instead of being repressed by glucose was, at least for long-chain fatty acids, stimulated by this metabolite.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study indicated that S.coelicolor strain M145 can effectively utilize fatty acids of different chain length, from C4 to C18, as sole carbon energy source.