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The acute toxicity of Methyl isothiocyanate was evaluated with Oncorhynchus mykiss in a study performed in accordance with OECD testing guideline 203 and GLP requirements. LC50-96 h was estimated using measured concentrations as being equal to 0.0531 mg/L.

The acute toxicity of Methyl isothiocyanate was evaluated with Daphnia magna in a study performed in accordance with OECD testing guideline 202 and GLP requirements.EC50-48 h was estimated using measured concentrations as being equal to 0.076 mg/L.

The acute toxicity of Methyl isothiocyanate was evaluated with Pseudokirschneriella subcapitata in a study performed in accordance with OECD testing guideline 201 and GLP requirements.ECr50-72 h was estimated using measured concentrations as being equal to 0.58 mg/L. (For information, ECb50-72 h was estimated equal to 0.28 mg/L).