2-isopropyl-N-hydroxyethyl 1,3-oxazolidine;methyl carbonato-N-ethyl-2-isopropyl-1,3-oxazolidine;reaction mass of: carbonato-bis-N-ethyl-2-isopropyl-1,3-oxazolidine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1998-09-01 to 1999-01-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study is regarded as relaible without restrictions because it was conducted in compliance with GLP regulaiton and guideline.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

cited as : Directive 92/69/EEC

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sampling method: Aqueous samples were taken from the test medium to determine the concentration of the major breakdown product of Incozol LV. Samples were taken at the start and end of the exposure period.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The 100 mg/L test medium was prepared by direct addition of Incozol LV to dilution water. The test medium was stirred using a magnetic stirrer for approximately 2 h before addition to the test vessels. Following addition of the D. magna the test vessels were sealed. A control treatment was prepared containing dilution water only.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: The test was carried out using a single clone of Daphnia magna cultured at Covance.
- Age at study initiation: <= 24 hours
- Food type: unicellular green algae, Ankistrodesmus falcatus (4E+07 cells/mL)
- Amount: 2 mL per vessel
- Frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

170 mg/L CaCO3

Test temperature:

19.4 ± 1 °C

pH:

Control : 7.0
100 mg/L : 8.0

Dissolved oxygen:

control : 98 (% air saturated value)
100 mg/L: 95 (% air saturated value)

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
No measured concentration.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass conical flask
- Type: closed
- Material, size, headspace, fill volume: containing approximately 120 mL of test medium when fully filled.
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daily 16 h photoperiod.

TEST CONCENTRATIONS
A limit test was conducted with 100 mg/L test item concentration.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The 48-h LC50 was determined to be greater then 100 mg/L and the 48-h NOEC was determined to be greater then 100 mg/L.

Executive summary:

A study was conducted according to OECD TG 202 and Directive 92/69/EEC method C.2 to determine the acute toxicity of Incozol LV to aquatic invertebrates. The study was conducted as limit test. Therefore, a solution with concentration of 100 mg/L test item was prepared by direct addition of Incozol LV to dilution water; the test medium was stirred for approximately 2 h before addition to the test vessels. Following addition of the D. magna the test vessels were sealed. A control treatment was prepared containing dilution water only. No immobility of D. magna was observed, accordingly, the 24- and 48-h EC50 values of Incozol LV were determined to be > 100 mg/L.

Description of key information

The acute toxicity of Incozol LV to Daphnia magna was determined after 48-h as LC50 to be greater than 100 mg/L and the 48-h NOEC was determined to be greter than 100 mg/L, respectively.

Additional information

A study was conducted according to OECD TG 202 and Directive 92/69/EEC method C.2 to determine the acute toxicity of Incozol LV to aquatic invertebrates. The study was conducted as limit test. Therefore, a solution with concentration of 100 mg/L test item was prepared by direct addition of Incozol LV to dilution water; the test medium was stirred for approximately 2 h before addition to the test vessels. Following addition of the D. magna the test vessels were sealed. A control treatment was prepared containing dilution water only. No immobility of D. magna was observed, accordingly, the 24- and 48-h EC50 values of Incozol LV were determined to be > 100 mg/L.