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Registration Dossier
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EC number: 255-288-2 | CAS number: 41272-40-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Additional information
Under standard test conditions Malachite Green (MG) did not show to be readily biodegradable (0.3%, 14 days, OECD 301C), while testing for inherent biodegradability resulted in 82% degradation presumably over a 54 day time span in the OECD 302B test (Dystar, 1998), which leads to the conclusion that MG is poorly biodegradable in water. Degradation rates with various microorganisms varied but can reach up to 90% degradation within several days, indicating the potential of microorganisms to rapidly degrade MG.
pH has a significant effect on the decolourization of MG, with an increase in decolourization when pH increases and a maximum at pH 9.
Biodegradation has mostly been tested by evaluating the decolourization of MG over time. In most cases the influence of sorption or pH change and subsequent decolourization has been noted but not taken into account in the conclusions or discussion of the studies. The mentioned interacting effects have sometimes been studied separately, but not in combination with each other; it can be only stated that combined effects usually contribute to improve the yield of degradation.
Since the primary source of environmental pollution of MG is represented by industrial waste water, many studies of biodegradability by a variety of organisms (bacteria, algae, fungi, ) were carried out, in order to find (micro)organisms resistant to MG and capable of turning MG into a non-toxic compound with less environmental impact for use in water treatment plants. Several common species of microorganisms have been identified which are able to degrade MG up to 90% within several days. Furthermore sludge coming from industrial specific waste water treatment are sent to incinerator.
Biodegradation in soil and sediment is not considered because direct and indirect exposure of soil and sediment is unlikely due to the risk management measures that will be applied at industrial level and discussed in details within the exposure scenario to avoid any release of the substance directly in the municipal waste water treatment.
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