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No reliable BCF was found; two studies report by Dystar, 1998, provides a BCF estimated from logPow of >36 - <91 for Malachite Green (MG) hydrochloride and states that no bioaccumulation is expected. This result is consistent with the proposal of the Committee for Risk Assessment RAC of no considering MG as a non bioaccumulative substance based on a logKow < 3.

Notwithstanding, after waterborne exposure of channel catfish to MG, MG and its metabolites can be traced in all fish tissues, with highest residues in adipose tissue and lowest residues in plasma. MG was rapidly and extensively metabolized to its reduced form, Leucomalachite Green, which was slowly eliminated from fish tissues (Plakas et al. 1995).

Bilandzic et al. (2010) studied residue levels of MG in carp and rainbow trout after treatment with MG in Croatian fish farms, using an in-house enzyme linked immunoassay (ELISA) validated to the criteria of Commission Decision 2002/657/EC with an LOD of 0.31 µg/kg. The highest concentration of MG residue was determined in rainbow trout at a concentration of 1.07 µg/kg.

Schuetze et al. (2008), determined residue levels of MG and LG in wild eels caught in catchment areas after municipal sewage treatment plants (STP) in Berlin, Germany. LG was the dominating residue with LG:MG ratios varying between 5:1 and 7:1. MG and its metabolite LG were detected with total concentrations up to 0.765 µg/ kg fresh weight in the tissues of 25 out of 45 eels caught from different lakes, a river and a canal. In all cases, the occurrence of the residues could directly be linked to the presence of discharges by municipal STPs into the receiving surface waters.


While no BAF/BCF values could be determined it is established that the bioaccumulation of MG and its metabolite LG in fish is possible, also due to municipal releases i.e. by wash off from clothes or paper towels coloured with MG.