3,5,5-trimethylhexanal

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP-Study according to OECD Guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(1992)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

(1992)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,5,5-trimethylhexanal

EC Number:

226-603-0

EC Name:

3,5,5-trimethylhexanal

Cas Number:

5435-64-3

Molecular formula:

C9H18O

IUPAC Name:

3,5,5-trimethylhexanal

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

For the preparation of a stock solution 1 g/l of the test substance was equilibrated in synthetic fresh water without NaHCO3,
stirred for about 18 h, and finally filtered of. The resulting solution served as stock solution. NaHCO3 was added after sampling for analytical verification. The test solutions were prepared daily.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Cyprinus carpio (Common carp)

Source: Bio International B.V., Someren, Netherlands

Size: 3 - 5 cm

Maintenance: The fish are maintained in dechlorinated drinking water in 200 l tanks or in 300 l glass tanks at 20 ° C +/- 2 ° C with continuous flow.
The daily amount of feed (Alma) is administered in an amount of approximately 2 % of the body weight. The animals are held in quarantine for
14 days. Only then, they are used in tests.

The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.

The mortality 7 days before test began was < 5 %.

Pretreatment: The test animals were quarantined for 14 days prior to the start of the test without any treatment.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

14 ° dH

Test temperature:

mean = 19.8 ° C
mind. 19.5 ° C/max. 20.3 ° C

pH:

7.8 - 8.9

Dissolved oxygen:

86 - 104 % saturation

Nominal and measured concentrations:

nominal and measured concentrations: 6 mg/L,11 mg/L, 20 mg/L, 34 mg/L, and 60 mg/L.

Details on test conditions:

Test tank: 20 l
Test volume: 10 l, changed daily
Aeration: continuous in the test tank
Light/dark cycle: 16/8 h
Density of animals: 10 animals per tank, each at ~ 0.91 g
Feed: none

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

range 34-60 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

The highest concentration at which no mortality occured.

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Remarks:

highest cocentration under the test conditions.

Details on results:

Analytical values of stability control, test solutions and stock solution. The concentrations are based on the substance. The measured test
concentrations after 0 h did not deviate by more than 20 % from the nominal concentrations. Additionally the measured concentration after 24 h
(first batch) did not deviate more than 20 % from the feshly prepared test solutions. Therefore it can be concluded that the test substance (or possible hydrolysis products) was present over the whole exposure time. The biological values were calculated with the nominal concentrations.

The highest concentration at which no mortality (< 10 %) after 96 h exposure occured was 96 h LC 0 = 34 mg/l.
The lowest concentration at which 100 % mortality after 96 h exposure occured, ws 96 LC 100 = 60 mg/l.

Any other information on results incl. tables

Sublethal observations / clinical signs:

s. attached test report

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

96 h LC50 = 45 mg/l (Concentration based on the substance)

Executive summary:

Test substance 3,5,5 -Trimethylhexanal

Test organism: Cyprinus carpio

Test type : Acute toxicity 96h LC50 according to OECD 203

96 h LC50 = 45 mg/l

(Concentration based on the test substance)