Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 449-400-0 | CAS number: 25822-43-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The test item was investigated for its toxicity to microorganisms following OECD 209. The test item did not inhibit respiration of activated slugde within the exposure period of 3 hours. The EC50 was estimated to be > 300 mg/L. The NOEC was determined to be 300 mg/L. The test item is considered to be not toxic to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 300 mg/L
- EC10 or NOEC for microorganisms:
- 300 mg/L
Additional information
The toxicity of the test item to microorganisms was assessed in a respiration inhibition test according to OECD 209, adopted in 1984. Five concentrations of the test substance (7.6, 19.8, 51.4, 123.2 and 300.8 mg/L) were compared to two negative controls (tap water). As positive control substance 3, 5-dichlorophenol was used and tested in three concentrations (5.0, 12.2 and 29.9 mg/L). As the test item was not soluble in water it was added directly to the samples with the activated sludge. The concentration of the freshly collected activated sludge was 1.6 g/L dry weight. The inoculum was kept aerated before the beginning of the test. After mixing the inoculum with synthetic sewage solution and appropriate amounts of control or test substances, the samples were aerated for a contact time of 3 hours at 18.5 °C. Negative controls were run as first and as last sample. After incubation the respiration rates were determined in closed bottles using an oxygen sensitive electrode. The inhibition of respiration was calculated from the respiration rates using the mean value of the negative controls as 100 %. The respiration rates of the two control samples were within 15 % of each other. The EC50 of 3, 5-dichlorophenol was in the accepted range of 5 to 30 mg/L. The test substance did not inhibit the respiration of the activated sludge. The highest obtained inhibition was 1.2 % which is less than the differences between the control samples. The EC50 was estimated to be > 300 mg/L. The NOEC was determined to be 300 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
