p,p'-(2-pyridylmethylene)bisphenol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-12-03 to 2002-12-06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted under GLP conditions in accordance with the official OECD guideline 202 (1984).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(1984)

Deviations:

yes

Remarks:

Part I only

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

(1992)

GLP compliance:

yes (incl. QA statement)

Remarks:

002/221/2002

Test material

Test material information

1

Reference substance name:

p,p'-(2-pyridylmethylene)bisphenol

EC Number:

210-039-7

EC Name:

p,p'-(2-pyridylmethylene)bisphenol

Cas Number:

603-41-8

Molecular formula:

C18H15NO2

IUPAC Name:

4-[(4-hydroxyphenyl)(pyridin-2-yl)methyl]phenol

Test material form:

solid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The test medium was prepared as follows: A supersaturated dispersion with a loading rate of 100 mg/L was prepared by weighing 29.9 mg of the test item into 300 ml test water. No auxiliary solvent or emulsifier was used. The test item was mixed into the test water as homogenously as possible by ultrasonic treatment for 15 minutes and by intense stirring for 48 hours at room temperature in the dark to dissolve a maximum concentration of the test item in the dispersion. Then, the supersaturated stock dispersion of the test item was filtered through a membrane filter under vacuum. The test medium was prepared just before the introduction of the daphnids. On request of the sponsor the concentrations of the test item were not analytically verified during the test period.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Daphnia magna, Straus - Strain: Straus- Source: University of Sheffield/UK- Age at study initiation (mean and range, SD): 6-24 hours- Method of breeding: Clone is bred in recontsituted water of the quality identical to the water quality used in the tests (regarding pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests.- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (=250 mg/L) as CaCO3

Test temperature:

20°C

pH:

7.8 to 7.9

Dissolved oxygen:

8.6 to 8.7 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

100 mg/L test item (nominal)

Details on test conditions:

TEST SYSTEM- Test vessel: 100 mL glass beakers, containing 50 ml medium- Type (delete if not applicable): covered with glass plate- Aeration: no - No. of organisms per vessel: 10 per vessel- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2- Biomass loading rate: the loading rate was lower than one daphnia per 2 mL test solutionTEST MEDIUM / WATER PARAMETERS- Test water: Reconstituted test water (Analytical grade salts were dissolved in purified water being saturated with oxygen prior to testing).OTHER TEST CONDITIONS- Photoperiod: 16-hour light and 8 hour dark- Light intensity: between 550 and 685 Lux

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

A limit test was performed in accordance with the EU Directive 92/69/EEC to demonstrate that the test item has no toxic effect on Daphnia magna up to the highest concentration which could be dissolved in the test water.

In the control and in the undiluted filtrate of the test item (with a loading rate of 100 mg/L) no immobilized test organisms or other signs of intoxication were determined during the test period of 48 hours.

Therefore, the 48 hour NOEC and the 48-hour EC0 of Oxypicoline RF to Daphnia magna were determined to be at least at a loading rate of 100 mg/L. The 48-hour NOEC and the 48-hour EC0 might even be higher, but concentrations in excess of the loading rate of 100 mg/L have not been tested due to the limited water solubility of the test item in the test water. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the loading rate of 100 mg/L. These values could not be quantified, since the test item had no toxic effect at a loading rate of 100 mg/L, and thus above the water solubility limit of the test item in the used test water.

Applicant's summary and conclusion

Conclusions:

In conclusion, the test item Oxypicoline RF had no acute toxic effects on Daphnia magna up to its solubility limit in test water under the present conditions.