O,O-diisopropyl hydrogen dithiophosphate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (1995) Part B. Protocol for a Fish Test Acute Toxicity Test

Deviations:

yes

Remarks:

Slightly bigger fish was used resulting in a weight load per test container volume(3.2-3.45 g/l) than recomended in guideline (1 g/l). This is considered to be of minor importance.

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Modified according to Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (1995) Part B.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

O,O-diisopropyl hydrogen dithiophosphate

EC Number:

203-503-5

EC Name:

O,O-diisopropyl hydrogen dithiophosphate

Cas Number:

107-56-2

Molecular formula:

C6H15O2PS2

IUPAC Name:

O,O-bis(propan-2-yl) sulfanylphosphonothioate

Test material form:

other: liquid

Details on test material:

- Analytical purity: 98 % w/w

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

- Concentrations:(nominial) 0, 10, 20, 50, 100 and 200 mg/l
- Sampling method: 40 ml taken out in 50 ml bottles of all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: Scophtalmus maximus

Details on test organisms:

TEST ORGANISM
- Common name: Turbot
- Source: Maximus in Thisted, Denmark
- Age at study initiation (mean and range, SD): Juvenile

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

14.5 ± 0.5 ºC

pH:

7.9 - 0.1

Dissolved oxygen:

>70 %

Salinity:

33 ‰

Nominal and measured concentrations:

Nominal concentrations: 0(control), 0, 10, 20, 50, 100 and 200 mg/l.

Details on test conditions:

- Test vessels: 20 l aquariums

- No. of organisms per vessel: 5

- Biomass loading rate: approx 3-4 g per l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12:12 (light:dark)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, CAS No. : 7778-50-9

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: yes
- LC50: 300-500 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50(96 h) of O,O-diisopropyl hydrogen dithiophosphate, CAS No.107-56-2, is above 200 mg/L for Scophtalmus maximus