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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006.07.10-2006.07.14
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: OSPAR Commission 2005: Protocols on Methods for the Testing of Chemicals used in the Offshore Industry (reference number: 2005-11 [a revised version of agreement 1995-07])
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Modified according to OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4'-difluorobenzophenone
EC Number:
206-441-7
EC Name:
2,4'-difluorobenzophenone
Cas Number:
342-25-6
Molecular formula:
C13H8F2O
IUPAC Name:
(2-fluorophenyl)(4-fluorophenyl)methanone
Details on test material:
2,4'-difluorbenzophenon
Liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: (Water accomodated fraction (WAF)): 20, 50, 100, 200, 500, 1000 ml WAF/l
- Sampling method: 2 x 50 ml of stock solution
- Sample storage conditions before analysis: Freezer at -21ºC

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Cyprinodon variegatus
Details on test organisms:
TEST ORGANISM
- Common name: Sheephead minnow
- Source: originally obtained from a stock culture at Opus Plus Ltd.(the former ERT Orkney water Technology Centre, and cultured at DHI since June 2003. Animals from this DHI culture was used for this study

ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from.

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20±0.5ºC
pH:
7.9-8.1
Dissolved oxygen:
> 70%
Salinity:
32 ‰
Nominal and measured concentrations:
Stock solution made by 1000 mg substance dissolved in 2000 ml test media. Nominal concentration: 500 mg/l.
Substance was not fully dissolved, therfore a test was taken from the middle of the volumetric flask (water accomodated fraction = WAF)
The stock solution was analysed at the beginning and by water replacement. Both times the analytical value was 37 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: 2 L aquariums containing 1 L test solution
- Renewal rate of test solution (frequency/flow rate): after 48 h.
- No. of organisms per vessel: 10
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5, at 2.3 mg/L.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
7.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: 50% at 2.3 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Mortality in controls < 10 %, oxygen > 60%, mortality with reference test within acceptable range, 20-80 %
Conclusions:
LC50(96 h) of 2,4'-difluorobenzophenone, CAS No. 342-25-6 is 7.10 mg/L for Cyprinodon variegatus