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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-08-16 - 2012-09-13
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD Guideline 301 D (Ready Biodegradabality: Closed Bottle Test)
Deviations:
no
Principles of method if other than guideline:
In biodegradation test OECD 301 D the oxygen consumption corresponded to 30.6% of the ThOD after 14 days in the toxicity control. Therefore the test item can be considered not inhibitory to the inoculum according to this test guideline
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: 51 % w/w solution in water, stabilized with X % NaOH
Details on test material:
- Substance type: Liquid
- Physical state: Light brown liquid
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES of test material
- Boiling point: 107 °C
- Water solubility (under test conditions): > 1000 g/L
- pH 12-14
- log Pow: 1.67

Test organisms

Test organisms (species):
sewage, domestic
Details on inoculum:
Secondary effluent from a municipal wastewater treatment plant in Usserød, Denmark, wihich predominantly receives domestic sewage

Study design

Total exposure duration:
28 d

Test conditions

Reference substance (positive control):
yes
Remarks:
benzoic acid, sodium salt

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
other: oxygen consumption
Effect conc.:
30.6 other: % of ThOD
Nominal / measured:
meas. (not specified)
Conc. based on:
other: oxygen elektrode
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In biodegradation test OECD 301 D the oxygen consumption corresponded to 30.6% of the ThOD after 14 days in the toxicity control. Therefore the tested substance can be considered not inhibitory to the inoculum according to this test guideline
Remark: The test solution contains NaOH due to the production method.